Immunology Antibodies 3

Phospho-LCK-Y394 Rabbit Polyclonal Antibody (CABP0182)

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SKU:
CABP0182
Product Type:
Antibody
Applications:
WB
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse
Synonyms:
LSK, YT16, IMD22, p56lck, pp58lck, Phospho-LCK-Y394
Research Use:
Immunology
Frequently bought together:

Description

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Phospho-LCK-Y394 Antibody (CABP0182)

The Phospho-LCK-Y394 Antibody (CABP0182) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the phosphorylated form of LCK at tyrosine 394, this antibody allows for the detection and analysis of LCK activation in various cell types, making it an essential tool for studies in immunology and cancer research.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:Phospho-LCK-Y394 Antibody
SKU:CABP0182
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:NEYT A
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:LSK, YT16, IMD22, p56lck, pp58lck, Phospho-LCK-Y394
Positive Sample:NIH/3T3 treated with Pervanadate, Jurkat
Cellular Localization:Cell Membrane, Cytoplasm, Cytoplasmic Side, Lipid-Anchor.
Calculated MW:58kDa
Observed MW:60kDa

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.

Purification MethodAffinity purification
Gene ID3932
RRIDAB_2771262
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Phospho-LCK-Y394 Rabbit pAb (CABP0182) at 1:1000 dilution. NIH/3T3 cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageWestern blot analysis of lysates from Jurkat cells, using Phospho-LCK-Y394 Rabbit pAb (CABP0182) at 1:1000 dilution. Jurkat cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
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