Epigenetics & Nuclear Signaling Antibodies 5
Anti-Phospho-Histone HIST1H3A-T3 pAb Antibody (CABP0846)
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- SKU:
- CABP0846
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-Phospho-Histone HIST1H3A-T3 Antibody |
| Antibody SKU: | CABP0846 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around T3 of human HIST1H3A (NP_003520.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Positive Samples: | HeLa, NIH/3T3, C6, 293T, RAW264.7 |
| Immunogen: | A synthetic phosphorylated peptide around T3 of human HIST1H3A (NP_003520.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MART K |
| Gene ID: | 8350 |
| Uniprot: | P68431 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 15kDa |
| Observed MW: | 17KDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T3 pAb (CABP0846) at 1:1000 dilution.C6 and HeLa cells were treated by Nocodazole (50 ng/mL) at 37'C for 20 hours.C6 and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T3 pAb (CABP0846) at 1:1000 dilution or Histone H3 antibody (CAB2348).Both 293T cells and Raw264.7 cells were treated by Nocodazole (100 ng/mL) at 37'C for 16 hours.C6 cells were treated by Nocodazole (50 ng/mL) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| Synonyms: | H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
| UniProt Protein Function: | H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.2 Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex Molecular Function:cadherin binding; histone binding; protein binding Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P68431 |
| NCBI GenInfo Identifier: | 55977055 |
| NCBI Gene ID: | 8357 |
| NCBI Accession: | P68431.2 |
| UniProt Secondary Accession: | P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1, |
| UniProt Related Accession: | P68431 |
| Molecular Weight: | 15kDa |
| NCBI Full Name: | Histone H3.1 |
| NCBI Synonym Full Names: | histone cluster 1 H3 family member h |
| NCBI Official Symbol: | HIST1H3H |
| NCBI Official Synonym Symbols: | H3/k; H3FK; H3F1K |
| NCBI Protein Information: | histone H3.1 |
| UniProt Protein Name: | Histone H3.1 |
| UniProt Synonym Protein Names: | Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l |
| UniProt Gene Name: | HIST1H3A |
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