Cell Biology Antibodies 14

Phospho-Histone H3.3-T3 Rabbit Monoclonal Antibody (CABP1152)

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SKU:
CABP1152
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Synonyms:
H3F3, H3-3B, H3.3A, H3F3A, BRYLIB1, Phospho-Histone H3.3-T3
Research Use:
Cell Biology
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Description

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Phospho-Histone H3.3-T3 Monoclonal Antibody (CABP1152)

The Phospho-Histone H3.3-T3 Monoclonal Antibody (CABP1152) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets phosphorylated Threonine 3 on Histone H3, a key event in the condensation of chromosomes during mitosis. Raised in rabbits, this antibody is highly sensitive and selective for detecting Histone H3 phosphorylation at T3, making it ideal for use in applications such as immunofluorescence and chromatin immunoprecipitation. Its high specificity allows for precise visualization and measurement of Histone H3 phosphorylation, providing valuable insights into the regulation of cell division and gene expression.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.

Product Name:Phospho-Histone H3.3-T3 Monoclonal Antibody
SKU:CABP1152
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Clone Number:ARC1662
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MART K
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:H3F3, H3-3B, H3.3A, H3F3A, BRYLIB1, Phospho-Histone H3.3-T3
Positive Sample:HeLa treated with Nocodazole, NIH/3T3 treated with Nocodazole, C6 treated with Nocodazole
Cellular Localization:Chromosome, Nucleus.
Calculated MW:15kDa
Observed MW:17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.

Purification MethodAffinity purification
Gene ID8290 8350
RRIDAB_2864016
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3.3-T3 Rabbit mAb (CABP1152) at 1:1000 dilution. NIH/3T3 cells were treated with Nocodazole (50 ng/mL) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-Histone H3.3-T3 Rabbit mAb (CABP1152) at 1:1000 dilution. HeLa cells were treated with nocodazole (50 ng/mL) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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