Cell Biology Antibodies 16

Anti-Phospho-EEF2K-S366 pAb Antibody (CABP0845)

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SKU:
CABP0845
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-Phospho-EEF2K-S366 Antibody
Antibody SKU:CABP0845
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, NIH/3T3, C6
Immunogen:A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TLSG S
Gene ID:29904
Uniprot:O00418
Cellular Location:
Calculated MW:82kDa
Observed MW:110kDa
Anti-Phospho-EEF2K-S366 pAb (CABP0845)Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 antibody (CABP0845) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-Phospho-EEF2K-S366 pAb (CABP0845)Western blot analysis of extracts of various cell lines, using Phospho-EEF2K-S366 antibody (CABP0845) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by CIP(20uL/400ul) at 37'C for 1 hour or PMA/TPA (200 nM) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-Phospho-EEF2K-S366 pAb (CABP0845)Western blot analysis of extracts of HeLa cells, using Anti-Phospho-EEF2K-S366 pAb (CABP0845) at 1:1000 dilution or EEF2K antibody (CAB5404).Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Synonyms:EEF2K, HSU93850, eEF-2K
Background:This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
UniProt Protein Function:EEF2K: an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Translation; Kinase, protein; Protein kinase, atypical; EC 2.7.11.20; ATYPICAL group; Alpha family; eEF2K subfamily

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: cytoplasm; cytosol

Molecular Function:calmodulin binding; elongation factor-2 kinase activity; translation factor activity, nucleic acid binding; calcium ion binding; ATP binding; protein kinase activity

Biological Process: translational elongation; regulation of protein amino acid autophosphorylation; protein amino acid autophosphorylation; insulin receptor signaling pathway

NCBI Summary:This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]
UniProt Code:O00418
NCBI GenInfo Identifier:254763289
NCBI Gene ID:29904
NCBI Accession:O00418.2
UniProt Secondary Accession:O00418,Q8N588,
UniProt Related Accession:O00418
Molecular Weight:725
NCBI Full Name:Eukaryotic elongation factor 2 kinase
NCBI Synonym Full Names:eukaryotic elongation factor 2 kinase
NCBI Official Symbol:EEF2K  
NCBI Official Synonym Symbols:eEF-2K; HSU93850  
NCBI Protein Information:eukaryotic elongation factor 2 kinase; eEF-2 kinase; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
UniProt Protein Name:Eukaryotic elongation factor 2 kinase
UniProt Synonym Protein Names:Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
Protein Family:Eukaryotic elongation factor 2 kinase
UniProt Gene Name:EEF2K  
UniProt Entry Name:EF2K_HUMAN
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