Cell Cycle Antibodies 2
Anti-Phospho-CDK6-Y13 Antibody (CABP0326)
- SKU:
- CABP0326
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-Phospho-CDK6-Y13 Antibody |
| Antibody SKU: | CABP0326 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y13 of human CDK6 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y13 of human CDK6 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 1021 |
| Uniprot: | Q00534 |
| Cellular Location: | Cell projection, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, ruffle |
| Calculated MW: | 36kDa |
| Observed MW: | 37kDa |
 | Western blot analysis of extracts from 293 cells using Phospho-CDK6-Y13 antibody (CABP0326). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
| Synonyms: | CDK6, MCPH12, PLSTIRE |
| Background: | The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| UniProt Protein Function: | CDK6: a protein kinase of the CDK family. Important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. Phosphorylates and regulates the activity of the Rb tumor suppressor protein. Overexpressed and/or disrupted by translocation in leukemias, lymphomas and other cancers and amplified in gliomas and rodent cancers. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.22; Protein kinase, CMGC; Cell cycle regulation; CMGC group; CDK family; CDK/CDK4 subfamily; CDK4 subfamily Chromosomal Location of Human Ortholog: 7q21-q22 Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytoplasm; cytosol; nucleoplasm; nucleus; ruffle Molecular Function:ATP binding; cyclin binding; cyclin-dependent protein kinase activity; protein binding Biological Process: astrocyte development; cell cycle arrest; cell dedifferentiation; dentate gyrus development; G1/S transition of mitotic cell cycle; generation of neurons; gliogenesis; lateral ventricle development; negative regulation of cell cycle; negative regulation of cell differentiation; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of myeloid cell differentiation; negative regulation of osteoblast differentiation; positive regulation of cell-matrix adhesion; positive regulation of fibroblast proliferation; protein amino acid phosphorylation; regulation of erythrocyte differentiation; regulation of gene expression; response to virus Disease: Microcephaly 12, Primary, Autosomal Recessive |
| NCBI Summary: | The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009] |
| UniProt Code: | Q00534 |
| NCBI GenInfo Identifier: | 223718134 |
| NCBI Gene ID: | 1021 |
| NCBI Accession: | NP_001138778.1 |
| UniProt Secondary Accession: | Q00534,A4D1G0, |
| UniProt Related Accession: | Q00534 |
| Molecular Weight: | 36,938 Da |
| NCBI Full Name: | cyclin-dependent kinase 6 |
| NCBI Synonym Full Names: | cyclin dependent kinase 6 |
| NCBI Official Symbol: | CDK6 |
| NCBI Official Synonym Symbols: | MCPH12; PLSTIRE |
| NCBI Protein Information: | cyclin-dependent kinase 6 |
| UniProt Protein Name: | Cyclin-dependent kinase 6 |
| UniProt Synonym Protein Names: | Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE |
| Protein Family: | Cyclin-dependent kinase |
| UniProt Gene Name: | CDK6 |
| UniProt Entry Name: | CDK6_HUMAN |
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