Cell Death Antibodies 2

Phospho-ABL1-Y412 Rabbit Polyclonal Antibody (CABP1060)

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SKU:
CABP1060
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
ABL, JTK7, p150, c-ABL, v-abl, CHDSKM, c-ABL1, BCR-ABL, bcr/abl, Phospho-c-Abl-Y412
Research Use:
Cell Death
Frequently bought together:

Description

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Phospho-ABL1-Y412 Antibody (CABP1060)

The Phospho-ABL1-Y412 Antibody (CABP1060) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically detects the phosphorylated form of ABL1 at tyrosine 412 and has been validated for use in Western blot applications. It enables the detection and analysis of phosphorylated ABL1 in human samples, providing insights into the regulation of cell signaling pathways.ABL1 is a key player in various cellular processes, including cell proliferation, differentiation, and migration.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Phospho-ABL1-Y412 Antibody
SKU:CABP1060
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:DTYT A
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ABL, JTK7, p150, c-ABL, v-abl, CHDSKM, c-ABL1, BCR-ABL, bcr/abl, Phospho-c-Abl-Y412
Positive Sample:C6
Cellular Localization:Cytoplasm, Lipid-Anchor, Mitochondrion, Nucleus, Nucleus Membrane, Cytoskeleton.
Calculated MW:123kDa
Observed MW:123kDa

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.

Purification MethodAffinity purification
Gene ID25
RRIDAB_2863932
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C6 cells, using Phospho-c-Abl-Y412 Rabbit pAb (CAB0282). C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-c-Abl-Y412 Rabbit pAb (CABP1060) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-c-Abl-Y412 Rabbit pAb (CABP1060) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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