Antibody Name:	Anti-PHD2 Antibody [KO Validated]
Antibody SKU:	CAB14557
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-426 of human PHD2 (NP_071334.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-426 of human PHD2 (NP_071334.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAND SGGP GGPS PSER DRQY CELC GKME NLLR CSRC RSSF YCCK EHQR QDWK KHKL VCQG SEGA LGHG VGPH QHSG PAPP AAVP PPRA GARE PRKA AARR DNAS GDAA KGKV KAKP PADP AAAA SPCR AAAG GQGS AVAA EAEP GKEE PPAR SSLF QEKA NLYP PSNT PGDA LSPG GGLR PNGQ TKPL PALK LALE YIVP CMNK HGIC VVDD FLGK ETGQ QIGD EVRA LHDT GKFT DGQL VSQK SDSS KDIR GDKI TWIE GKEP GCET IGLL MSSM DDLI RHCN GKLG SYKI NGRT KAMV ACYP GNGT GYVR HVDN PNGD GRCV TCIY YLNK DWDA KVSG GILR IFPE GKAQ FADI EPKF DRLL FFWS DRRN PHEV QPAY ATRY AITV WYFD ADER ARAK VKYL TGEK GVRV ELNK PSDS VGKD VF
Gene ID:	54583
Uniprot:	Q9GZT9
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	36kDa/43kDa/46kDa
Observed MW:	46kDa

Synonyms:

EGLN1, C1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6

Background:

The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).

UniProt Protein Function:	EGLN1: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Monomer. Interacts with ING4; the interaction inhibits the hydroxylation of HIFs. Interacts with LIMD1. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, TCEB2 AND CUL2. Interacts with EPAS1. According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. According to PubMed:12788921; expressed in adult and fetal heart, brain, liver, lung, skeletal muscle and kidney. Also expressed in placenta. Highest levels in adult heart, brain, lung and liver and fetal brain, heart spleen and skeletal muscle. Following exposure to hypoxia, activated in HeLa cells but not in cardiovascular cells. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 1.14.11.29; Oxidoreductase Chromosomal Location of Human Ortholog: 1q42.1 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:peptidyl-proline dioxygenase activity; protein binding; enzyme binding; L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; peptidyl-proline 4-dioxygenase activity Biological Process: oxygen homeostasis; negative regulation of cAMP catabolic process; negative regulation of transcription factor activity; cardiac muscle morphogensis; response to hypoxia; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; negative regulation of cyclic-nucleotide phosphodiesterase activity; regulation of angiogenesis Disease: Erythrocytosis, Familial, 3; Hemoglobin, High Altitude Adaptation
NCBI Summary:	The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov 2009]
UniProt Code:	Q9GZT9
NCBI GenInfo Identifier:	32129514
NCBI Gene ID:	54583
NCBI Accession:	Q9GZT9.1
UniProt Related Accession:	Q9GZT9
Molecular Weight:
NCBI Full Name:	Egl nine homolog 1
NCBI Synonym Full Names:	egl-9 family hypoxia inducible factor 1
NCBI Official Symbol:	EGLN1
NCBI Official Synonym Symbols:	HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2
NCBI Protein Information:	egl nine homolog 1
UniProt Protein Name:	Egl nine homolog 1
UniProt Synonym Protein Names:	Hypoxia-inducible factor prolyl hydroxylase 2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH-2; Prolyl hydroxylase domain-containing protein 2; PHD2; SM-20
UniProt Gene Name:	EGLN1
UniProt Entry Name:	EGLN1_HUMAN

	Western blot analysis of extracts of various cell lines, using PHD2 antibody (CAB14557) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of various cell lines, using PHD2 antibody (CAB14557) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and PHD2 knockout (KO) 293T cells, using PHD2 antibody (CAB14557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded Rat kidney using PHD2 antibody (CAB14557) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human colon using PHD2 antibody (CAB14557) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-PHD2 Antibody (CAB2314)	Human PHD2 (Prolyl hydroxylase domain-containing protein 2) ELISA Kit (HUFI08641)
EGLN1 Antibody (PACO09016)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)