Epigenetics & Nuclear Signaling Antibodies 1
Anti-PHB Antibody (CAB0056)
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- SKU:
- CAB0056
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PHB Antibody |
| Antibody SKU: | CAB0056 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 73-272 of human PHB (NP_002625.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, NIH/3T3, HeLa, MCF7, HepG2, Mouse liver, Mouse heart, Mouse kidney, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 73-272 of human PHB (NP_002625.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PRNV PVIT GSKD LQNV NITL RILF RPVA SQLP RIFT SIGE DYDE RVLP SITT EILK SVVA RFDA GELI TQRE LVSR QVSD DLTE RAAT FGLI LDDV SLTH LTFG KEFT EAVE AKQV AQQE AERA RFVV EKAE QQKK AAII SAEG DSKA AELI ANSL ATAG DGLI ELRK LEAA EDIA YQLS RSRN ITYL PAGQ SVLL QLPQ |
| Gene ID: | 5245 |
| Uniprot: | P35232 |
| Cellular Location: | Mitochondrion inner membrane |
| Calculated MW: | 17kDa/29kDa |
| Observed MW: | 30kDa |
| Synonyms: | HEL-215, HEL-S-54e, PHB1, PHB, Prohibitin |
| Background: | This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer. |
| UniProt Protein Details: | Protein type:Oncoprotein; Cell cycle regulation; Mitochondrial; Chaperone; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Apoptosis Chromosomal Location of Human Ortholog: 17q21 Cellular Component: nucleoplasm; membrane; mitochondrion; integral to plasma membrane; mitochondrial inner membrane; cytoplasm; nucleus Molecular Function:protein binding; enzyme binding; histone deacetylase binding Biological Process: osteoblast differentiation; regulation of apoptosis; transcription from RNA polymerase II promoter; negative regulation of cell proliferation; progesterone receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription, DNA-dependent Disease: Breast Cancer |
| NCBI Summary: | This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | P35232 |
| NCBI GenInfo Identifier: | 464371 |
| NCBI Gene ID: | 5245 |
| NCBI Accession: | P35232.1 |
| UniProt Secondary Accession: | P35232,Q4VBQ0, B4DY47, |
| UniProt Related Accession: | P35232 |
| Molecular Weight: | 272 |
| NCBI Full Name: | Prohibitin |
| NCBI Synonym Full Names: | prohibitin |
| NCBI Official Symbol: | PHBÂ Â |
| NCBI Official Synonym Symbols: | PHB1; HEL-215; HEL-S-54e  |
| NCBI Protein Information: | prohibitin; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e |
| UniProt Protein Name: | Prohibitin |
| Protein Family: | Prohibitin |
| UniProt Gene Name: | PHBÂ Â |
| UniProt Entry Name: | PHB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PHB antibody (CAB0056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using PHB Rabbit pAb (CAB0056) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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