Metabolism Antibodies 2
Anti-PGM1 Antibody (CAB6303)
- SKU:
- CAB6303
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PGM1 Antibody |
| Antibody SKU: | CAB6303 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PGM1 (NP_002624.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, HepG2, 293T, Mouse liver, Mouse heart, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PGM1 (NP_002624.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVKI VTVK TQAY QDQK PGTS GLRK RVKV FQSS ANYA ENFI QSII STVE PAQR QEAT LVVG GDGR FYMK EAIQ LIAR IAAA NGIG RLVI GQNG ILST PAVS CIIR KIKA IGGI ILTA SHNP GGPN GDFG IKFN ISNG GPAP EAIT DKIF QISK TIEE YAVC PDLK VDLG VLGK QQFD LENK FKPF TVEI VDSV EAYA TMLR SIFD FSAL KELL SGPN RLKI RIDA MHGV VGPY VKKI LCEE LGAP ANSA VNCV PLED FGGH HPDP NLTY AADL VETM KSGE HDFG AAFD GDGD RNMI LGKH |
| Gene ID: | 5236 |
| Uniprot: | P36871 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 40kDa/61kDa/63kDa |
| Observed MW: | 61kDa |
| Synonyms: | PGM1, CDG1T, GSD14 |
| Background: | The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene. |
| UniProt Protein Function: | This enzyme participates in both the breakdown and synthesis of glucose. |
| NCBI Summary: | The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010] |
| UniProt Code: | P36871 |
| NCBI GenInfo Identifier: | 585670 |
| NCBI Gene ID: | 5236 |
| NCBI Accession: | P36871.3 |
| UniProt Secondary Accession: | P36871,Q16105, Q16106, Q5BKZ9, Q6NW22, Q86U74, Q96J40 Q9NTY4, B2R5N9, B4DPV0, |
| UniProt Related Accession: | P36871 |
| Molecular Weight: | 40,283 Da |
| NCBI Full Name: | Phosphoglucomutase-1 |
| NCBI Synonym Full Names: | phosphoglucomutase 1 |
| NCBI Official Symbol: | PGM1 |
| NCBI Official Synonym Symbols: | CDG1T; GSD14 |
| NCBI Protein Information: | phosphoglucomutase-1 |
| UniProt Protein Name: | Phosphoglucomutase-1 |
| UniProt Synonym Protein Names: | Glucose phosphomutase 1 |
| Protein Family: | Phosphoglucomutase |
| UniProt Gene Name: | PGM1 |
| UniProt Entry Name: | PGM1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PGM1 antibody (CAB6303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of U2OS cells using PGM1 antibody (CAB6303). Blue: DAPI for nuclear staining. |
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