Anti-PFKP Antibody (CAB20983)
- SKU:
- CAB20983
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-PFKP Antibody (CAB20983) |
| Antibody SKU: | CAB20983 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 336-540 of human PFKP (NP_002618.1). |
| Application: | Western blotting, Immunohistochemistry |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, MCF7, Jurkat, HUH-7, Rat testis, Mouse brain, Rat brain, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 336-540 of human PFKP (NP_002618.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ATPD TPAC VVSL NGNH AVRL PLME CVQM TQDV QKAM DERR FQDA VRLR GRSF AGNL NTYK RLAI KLPD DQIP KTNC NVAV INVG APAA GMNA AVRS AVRV GIAD GHRM LAIY DGFD GFAK GQIK EIGW TDVG GWTG QGGS ILGT KRVL PGKY LEEI ATQM RTHS INAL LIIG GFEA YLGL LELS AARE KHEE FCVP MVMV PATV S |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 85kDa |
| Observed MW: | 86KDa |
| Synonyms: | PFKP, ATP-PFK, PFK-C, PFK-P, PFKF |
| Background: | This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using PFKP antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded rat kidney using PFKP antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using PFKP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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