Metabolism Antibodies 1
Anti-PFKP Antibody (CAB12160)
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- SKU:
- CAB12160
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PFKP Antibody |
| Antibody SKU: | CAB12160 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 605-784 of human PFKP (NP_002618.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-87MG, HeLa, BxPC-3, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 605-784 of human PFKP (NP_002618.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IFEE PFDI RDLQ SNVE HLTE KMKT TIQR GLVL RNES CSEN YTTD FIYQ LYSE EGKG VFDC RKNV LGHM QQGG APSP FDRN FGTK ISAR AMEW ITAK LKEA RGRG KKFT TDDS ICVL GISK RNVI FQPV AELK KQTD FEHR IPKE QWWL KLRP LMKI LAKY KASY DVSD SGQL EHVQ PWSV |
| Gene ID: | 5214 |
| Uniprot: | Q01813 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 85kDa |
| Observed MW: | 86kDa |
| Synonyms: | PFKP, ATP-PFK, PFK-C, PFK-P, PFKF |
| Background: | This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - galactose; Kinase, other; Carbohydrate Metabolism - fructose and mannose Chromosomal Location of Human Ortholog: 10p15.3-p15.2 Cellular Component: cytosol; membrane; nucleus Molecular Function:6-phosphofructokinase activity; protein complex binding |
| NCBI Summary: | The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q01813 |
| NCBI GenInfo Identifier: | 1346355 |
| NCBI Gene ID: | 5214 |
| NCBI Accession: | Q01813.2 |
| UniProt Secondary Accession: | Q01813,Q5VSR7, Q5VSR8, B3KS15, |
| UniProt Related Accession: | Q01813 |
| Molecular Weight: | 85596 Da |
| NCBI Full Name: | ATP-dependent 6-phosphofructokinase, platelet type |
| NCBI Synonym Full Names: | phosphofructokinase, platelet |
| NCBI Official Symbol: | PFKP |
| NCBI Official Synonym Symbols: | PFKF; PFK-C; PFK-P; ATP-PFK |
| NCBI Protein Information: | ATP-dependent 6-phosphofructokinase, platelet type |
| UniProt Protein Name: | ATP-dependent 6-phosphofructokinase, platelet type |
| UniProt Synonym Protein Names: | 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphohexokinase |
| Protein Family: | ATP-dependent 6-phosphofructokinase |
| UniProt Gene Name: | PFKP |
| UniProt Entry Name: | PFKAP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PFKP antibody (CAB12160) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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