Cell Biology Antibodies 1

Anti-Perforin Antibody (CAB0093)

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SKU:
CAB0093
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-Perforin Antibody
Antibody SKU:CAB0093
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human Perforin (NP_001076585.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:NIH/3T3, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human Perforin (NP_001076585.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EEKK KKHK MTAS FHQT YRER HSEV VGGH HTSI NDLL FGIQ AGPE QYSA WVNS LPGS PGLV DYTL EPLH VLLD SQDP RREA LRRA LSQY LTDR ARWR DCSR PCPP GRQK SPRD PCQC VCHG SAVT TQDC CPRQ RGLA QLEV TFIQ AWGL WGDW FTAT DAYV KLFF GGQE LRTS TVWD NNNP IWSV RLDF GDVL LATG GPLR LQVW DQDS GRDD DLLG TCDQ APKS GSHE VRCN LNHG HLKF RYHA RCLP HLGG GTCL DYVP QMLL GEPP GNRS GAVW
Gene ID:5551
Uniprot:P14222
Cellular Location:Cell membrane, Cytoplasmic granule lumen, Endosome lumen, Multi-pass membrane protein, Secreted
Calculated MW:61kDa
Observed MW:70KDa
Synonyms:PRF1, FLH2, HPLH2, P1, PFN1, PFP, perforin-1, Perforin
Background:The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein.
UniProt Protein Function:PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: membrane; cytoplasmic membrane-bound vesicle; integral to membrane; extracellular region; plasma membrane

Molecular Function:protein binding; calcium ion binding; wide pore channel activity

Biological Process: formation of immunological synapse; apoptosis; cytolysis; defense response to tumor cell; cellular defense response; immune response to tumor cell; defense response to virus; transmembrane transport; protein homooligomerization

Disease: Lymphoma, Non-hodgkin, Familial; Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2

NCBI Summary:The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
UniProt Code:P14222
NCBI GenInfo Identifier:129819
NCBI Gene ID:5551
NCBI Accession:P14222.1
UniProt Secondary Accession:P14222,Q59F57, Q86WX7, B2R6X4,
UniProt Related Accession:P14222
Molecular Weight:555
NCBI Full Name:Perforin-1
NCBI Synonym Full Names:perforin 1 (pore forming protein)
NCBI Official Symbol:PRF1  
NCBI Official Synonym Symbols:P1; PFP; FLH2; PFN1; HPLH2  
NCBI Protein Information:perforin-1; cytolysin; lymphocyte pore forming protein; lymphocyte pore-forming protein
UniProt Protein Name:Perforin-1
UniProt Synonym Protein Names:Cytolysin; Lymphocyte pore-forming protein; PFP
Protein Family:Perforin
UniProt Gene Name:PRF1  
UniProt Entry Name:PERF_HUMAN
Anti-Perforin Antibody (CAB0093)Western blot analysis of extracts of various cell lines, using Perforin antibody (CAB0093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-Perforin Antibody (CAB0093)Immunohistochemistry of paraffin-embedded mouse spleen using Perforin antibody (CAB0093) at dilution of 1:100 (40x lens).
Anti-Perforin Antibody (CAB0093)Immunohistochemistry of paraffin-embedded rat lung using Perforin antibody (CAB0093) at dilution of 1:100 (40x lens).
Anti-Perforin Antibody (CAB0093)Immunofluorescence analysis of NIH/3T3 cells using Perforin antibody (CAB0093) at dilution of 1:100. Blue: DAPI for nuclear staining.
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