Cell Biology Antibodies 10
Anti-PDXK Antibody (CAB6687)
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- SKU:
- CAB6687
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PDXK Antibody |
| Antibody SKU: | CAB6687 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human PDXK (NP_003672.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HT-29, HeLa, HepG2, Mouse liver, Mouse kidney, Mouse brain, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human PDXK (NP_003672.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEEE CRVL SIQS HVIR GYVG NRAA TFPL QVLG FEID AVNS VQFS NHTG YAHW KGQV LNSD ELQE LYEG LRLN NMNK YDYV LTGY TRDK SFLA MVVD IVQE LKQQ NPRL VYVC DPVL GDKW DGEG SMYV PEDL LPVY KEKV VPLA DIIT PNQF EAEL LSGR KIHS QEEA LRVM DMLH SMGP DTVV ITSS DLPS PQGS NYLI VLGS QRRR NPAG SVVM ERIR MDIR KVDA VFVG TGDL FAAM LLAW THKH PNNL KVAC EKTV STLH HVLQ RTIQ CAKA QAGE GVRP SPMQ LELR MVQS KRDI EDPE IVVQ ATVL |
| Gene ID: | 8566 |
| Uniprot: | O00764 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 26kDa/31kDa/35kDa |
| Observed MW: | 35kDa |
| Synonyms: | PDXK, C21orf124, C21orf97, HEL-S-1a, PKH, PNK, PRED79 |
| Background: | The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. |
| UniProt Protein Function: | PDXK: Required for synthesis of pyridoxal-5-phosphate from vitamin B6. Belongs to the pyridoxine kinase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Kinase, other; Cofactor and Vitamin Metabolism - vitamin B6; EC 2.7.1.35 Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:ATP binding; lithium ion binding; magnesium ion binding; potassium ion binding; protein homodimerization activity; pyridoxal kinase activity; pyridoxal phosphate binding; sodium ion binding; zinc ion binding Biological Process: cell proliferation; pyridoxal 5'-phosphate salvage; pyridoxal phosphate biosynthetic process; vitamin B6 metabolic process |
| NCBI Summary: | The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00764 |
| NCBI GenInfo Identifier: | 2811007 |
| NCBI Gene ID: | 8566 |
| NCBI Accession: | O00764.1 |
| UniProt Secondary Accession: | O00764,Q7Z2Y0, Q9BS02, |
| UniProt Related Accession: | O00764 |
| Molecular Weight: | 26,827 Da |
| NCBI Full Name: | Pyridoxal kinase |
| NCBI Synonym Full Names: | pyridoxal (pyridoxine, vitamin B6) kinase |
| NCBI Official Symbol: | PDXK |
| NCBI Official Synonym Symbols: | PKH; PNK; PRED79; C21orf97; HEL-S-1a; C21orf124 |
| NCBI Protein Information: | pyridoxal kinase |
| UniProt Protein Name: | Pyridoxal kinase |
| UniProt Synonym Protein Names: | Pyridoxine kinase |
| Protein Family: | Pyridoxine kinase |
| UniProt Gene Name: | PDXK |
| UniProt Entry Name: | PDXK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDXK antibody (CAB6687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of U2OS cells using PDXK antibody (CAB6687). Blue: DAPI for nuclear staining. |
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