Cell Death Antibodies 2

Anti-PCSK9 Antibody (CAB7860)

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SKU:
CAB7860
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-PCSK9 Antibody
Antibody SKU:CAB7860
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human PCSK9 (NP_777596.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human PCSK9 (NP_777596.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CCHA PGLE CKVK EHGI PAPQ EQVT VACE EGWT LTGC SALP GTSH VLGA YAVD NTCV VRSR DVST TGST SEGA VTAV AICC RSRH LAQA SQEL Q
Gene ID:255738
Uniprot:Q8NBP7
Cellular Location:Cell surface, Cytoplasm, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Secreted
Calculated MW:20kDa/74kDa
Observed MW:65KDa/80KDa
Synonyms:PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9
Background:This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PCSK9: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. Defects in PCSK9 are the cause of hypercholesterolemia autosomal dominant type 3 (HCHOLA3). A familial condition characterized by elevated circulating cholesterol contained in either low-density lipoproteins alone or also in very-low-density lipoproteins. Belongs to the peptidase S8 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; EC 3.4.21.-; Protease; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; rough endoplasmic reticulum; lysosome; endoplasmic reticulum; early endosome; ER to Golgi transport vesicle; extrinsic to external side of plasma membrane; perinuclear region of cytoplasm; cytoplasm; late endosome; plasma membrane

Molecular Function:sodium channel inhibitor activity; very-low-density lipoprotein binding; protein binding; protein self-association; low-density lipoprotein receptor binding; serine-type endopeptidase activity; low-density lipoprotein binding; apolipoprotein binding; apolipoprotein receptor binding

Biological Process: cholesterol metabolic process; lysosomal transport; apoptosis; positive regulation of receptor internalization; lipoprotein metabolic process; regulation of low-density lipoprotein receptor catabolic process; liver development; cellular response to starvation; proteolysis; neuron differentiation; protein autoprocessing; cholesterol homeostasis; triacylglycerol metabolic process; cellular response to insulin stimulus; neurogenesis; phospholipid metabolic process; positive regulation of neuron apoptosis; regulation of neuron apoptosis; negative regulation of receptor recycling; low-density lipoprotein receptor catabolic process; regulation of receptor activity; kidney development

Disease: Hypercholesterolemia, Autosomal Dominant, 3

NCBI Summary:This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:Q8NBP7
NCBI GenInfo Identifier:317373487
NCBI Gene ID:255738
NCBI Accession:Q8NBP7.3
UniProt Secondary Accession:Q8NBP7,Q5PSM5, Q5SZQ2, A8T640, C0JYY9,
UniProt Related Accession:Q8NBP7
Molecular Weight:20,827 Da
NCBI Full Name:Proprotein convertase subtilisin/kexin type 9
NCBI Synonym Full Names:proprotein convertase subtilisin/kexin type 9
NCBI Official Symbol:PCSK9  
NCBI Official Synonym Symbols:FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3  
NCBI Protein Information:proprotein convertase subtilisin/kexin type 9; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisin/kexin type 9 preproprotein
UniProt Protein Name:Proprotein convertase subtilisin/kexin type 9
UniProt Synonym Protein Names:Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin/kexin-like protease PC9
Protein Family:Proprotein convertase subtilisin/kexin
UniProt Gene Name:PCSK9  
UniProt Entry Name:PCSK9_HUMAN
Anti-PCSK9 Antibody (CAB7860)Western blot analysis of extracts of various cell lines, using PCSK9 antibody (CAB7860) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PCSK9 Antibody (CAB7860)Immunohistochemistry of paraffin-embedded rat kidney using PCSK9 antibody (CAB7860) at dilution of 1:100 (40x lens).
Anti-PCSK9 Antibody (CAB7860)Immunohistochemistry of paraffin-embedded human stomach using PCSK9 antibody (CAB7860) at dilution of 1:100 (40x lens).
Anti-PCSK9 Antibody (CAB7860)Immunohistochemistry of paraffin-embedded mouse liver using PCSK9 antibody (CAB7860) at dilution of 1:100 (40x lens).
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