Metabolism Antibodies 2

Pyruvate carboxylase (PC) Rabbit Polyclonal Antibody (CAB6301)

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SKU:
CAB6301
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
PCB, Pyruvate carboxylase (PC)
Research Use:
Metabolism
Frequently bought together:

Description

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Pyruvate carboxylase (PC) Antibody (CAB6301)

The Pyruvate carboxylase (PC) Antibody (CAB6301) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the CD300A protein, the antibody enables detection and analysis in various cell types, making it ideal for studies in immunology and cancer research.CD300A, also known as an immune inhibitory receptor, plays a critical role in immune homeostasis by regulating inflammation and inhibiting allergic reactions.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Pyruvate carboxylase (PC) Antibody
SKU:CAB6301
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:HSMG LGSK FKEV KKAY VEAN QMLG DLIK VTPS SKIV GDLA QFMV QNGL SRAE AEAQ AEEL SFPR SVVE FLQG YIGV PHGG FPEP FRSK VLKD LPRV EGRP GASL PPLD LQAL EKEL VDRH GEEV TPED VLSA AMYP DVFA HFKD FTAT FGPL DSLN TRLF LQGP KIAE EFEV ELER GKTL HIKA LAVS DLNR AGQR QVFF ELNG QLRS ILVK DTQA MKEM HFHP KALK DVKG QIGA PMPG KVID IKVV AGAK VAKG QPLC VLSA MKME TVVT SPME GTVR KVHV TKDM TLEG DDLI LEIE
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:10 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PCB, Pyruvate carboxylase (PC)
Positive Sample:HepG2, HeLa
Cellular Localization:Mitochondrion Matrix.
Calculated MW:130kDa
Observed MW:130kDa

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.

Purification MethodAffinity purification
Gene ID5091
RRIDAB_2766906
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using Pyruvate carboxylase (PC) Rabbit pAb (CAB6301) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunofluorescence analysis of U2OS cells using Pyruvate carboxylase (PC) Rabbit pAb (CAB6301). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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