Epigenetics & Nuclear Signaling Antibodies 1

Anti-PATZ1 Antibody (CAB10053)

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SKU:
CAB10053
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-PATZ1 Antibody
Antibody SKU:CAB10053
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human PATZ1 (NP_114440.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:4000
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, HeLa, MCF7, HL-60, HepG2, Mouse kidney, Mouse spleen, Mouse lung, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human PATZ1 (NP_114440.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PFPS VASS APPL TGKR GRGR PRKA NLLD SMFG SPGG LREA GILP CGLC GKVF TDAN RLRQ HEAQ HGVT SLQL GYID LPPP RLGE NGLP ISED PDGP RKRS R
Gene ID:23598
Uniprot:Q9HBE1
Cellular Location:Nucleus
Calculated MW:57kDa/69kDa/74kDa
Observed MW:74kDa
Synonyms:PATZ1, MAZR, PATZ, RIAZ, ZBTB19, ZNF278, ZSG, dJ400N23
Background:The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene.
UniProt Protein Function:PATZ1: Transcriptional repressor. A chromosomal aberration involving PATZ1 is associated with small round cell sarcoma. Translocation t(1;22)(p36.1;q12) with EWSR1. Belongs to the krueppel C2H2-type zinc-finger protein family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; C2H2-type zinc finger protein; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: nucleoplasm; nucleus

Molecular Function:chromatin binding; DNA binding; metal ion binding

Biological Process: male gonad development; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; spermatogenesis; T cell differentiation; transcription from RNA polymerase II promoter

NCBI Summary:The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9HBE1
NCBI GenInfo Identifier:14670360
NCBI Gene ID:23598
NCBI Accession:NP_055138.2
UniProt Secondary Accession:Q9HBE1,Q9HBE2, Q9HBE3, Q9P1A9, Q9UDU0, Q9Y529,
UniProt Related Accession:Q9HBE1
Molecular Weight:
NCBI Full Name:POZ-, AT hook-, and zinc finger-containing protein 1 long C isoform
NCBI Synonym Full Names:POZ/BTB and AT hook containing zinc finger 1
NCBI Official Symbol:PATZ1  
NCBI Official Synonym Symbols:ZSG; MAZR; PATZ; RIAZ; ZBTB19; ZNF278; dJ400N23  
NCBI Protein Information:POZ-, AT hook-, and zinc finger-containing protein 1
UniProt Protein Name:POZ-, AT hook-, and zinc finger-containing protein 1
UniProt Synonym Protein Names:BTB/POZ domain zinc finger transcription factor; Protein kinase A RI subunit alpha-associated protein; Zinc finger and BTB domain-containing protein 19; Zinc finger protein 278; Zinc finger sarcoma gene protein
Protein Family:POZ-, AT hook-, and zinc finger-containing protein
UniProt Gene Name:PATZ1  
UniProt Entry Name:PATZ1_HUMAN
Anti-PATZ1 Antibody (CAB10053)Western blot analysis of extracts of various cell lines, using PATZ1 antibody (CAB10053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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