Epigenetics & Nuclear Signaling Antibodies 1
Anti-PAPD7 Antibody (CAB10017)
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- SKU:
- CAB10017
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PAPD7 Antibody |
| Antibody SKU: | CAB10017 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 303-542 of human PAPD7 (NP_008930.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, A-549, SW620, HeLa, Mouse liver, Mouse brain, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 303-542 of human PAPD7 (NP_008930.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GSKA HPSP GMDS RIKI KERI ATCN GEQT QNRE PESP YGQR LTLS LSSP QLLS SGSS ASSV SSLS GSDV DSDT PPCT TPSV YQFS LQAP APLM AGLP TALP MPSG KPQP TTSR TLIM TTNN QTRF TIPP PTLG VAPV PCRQ AGVE GTAS LKAV HHMS SPAI PSAS PNPL SSPH LYHK QHNG MKLS MKGS HGHT QGGG YSSV GSGG VRPP VGNR GHHQ YNRT GWRR KKHT HTRD SLPV SLSR |
| Gene ID: | 11044 |
| Uniprot: | Q5XG87 |
| Cellular Location: | Cytoplasm, Nucleus, nucleoplasm |
| Calculated MW: | 59kDa/82kDa |
| Observed MW: | 82kDa |
| Synonyms: | PAPD7, LAK-1, LAK1, POLK, POLS, TRF4, TRF4-1, TRF41, TUTASE5 |
| Background: | The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. Alternatively spliced transcript variants that encode different isoforms have been described. |
| UniProt Protein Function: | POLS: DNA polymerase, probably involved in DNA repair. May play a role in sister chromatid cohesion. Does not play a role in replication-dependent histone mRNA degradation. Belongs to the DNA polymerase type-B-like family. |
| UniProt Protein Details: | Protein type:DNA replication; Transferase; DNA repair, damage; EC 2.7.7.19 Chromosomal Location of Human Ortholog: 5p15 Cellular Component: nucleus Molecular Function:SMC protein binding Biological Process: response to drug; sister chromatid cohesion |
| NCBI Summary: | The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jan 2010] |
| UniProt Code: | Q5XG87 |
| NCBI GenInfo Identifier: | 586830523 |
| NCBI Gene ID: | 11044 |
| NCBI Accession: | Q5XG87.3 |
| UniProt Secondary Accession: | Q5XG87,O43289, Q17RZ1, Q9Y6C1, A8K1E2, M1JCE6, |
| UniProt Related Accession: | Q5XG87 |
| Molecular Weight: | 59,874 Da |
| NCBI Full Name: | Non-canonical poly(A) RNA polymerase PAPD7 |
| NCBI Synonym Full Names: | poly(A) RNA polymerase D7, non-canonical |
| NCBI Official Symbol: | PAPD7 |
| NCBI Official Synonym Symbols: | LAK1; POLK; POLS; TRF4; LAK-1; TRF41; TRF4-1; TUTASE5 |
| NCBI Protein Information: | non-canonical poly(A) RNA polymerase PAPD7 |
| UniProt Protein Name: | Non-canonical poly(A) RNA polymerase PAPD7 |
| UniProt Synonym Protein Names: | DNA polymerase sigma; LAK-1; PAP-associated domain-containing protein 7; TRAMP-like complex polyadenylate polymerase; Terminal uridylyltransferase 5; TUTase 5; Topoisomerase-related function protein 4-1; TRF4-1 |
| UniProt Gene Name: | PAPD7 |
| UniProt Entry Name: | PAPD7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PAPD7 antibody (CAB10017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human placenta using PAPD7 Rabbit pAb (CAB10017) at dilution of 1:50 (40x lens). |
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