Anti-pan-Tri-Methyl lysine Antibody (CAB20145)
- SKU:
- CAB20145
- Product Type:
- Antibody
- Reactivity:
- Universal
- Antibody Type:
- Polyclonal Antibody
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- Applications:
- IP
- Applications:
- ChIP
Description
| Product Name: | pan-Tri-Methyl lysine Rabbit pAb |
| Product Code: | CAB20145 |
| Size: | 50uL, 100uL |
| Applications: | WB, IP, ChIP |
| Reactivity: | ALL |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence containing Tri-methylated K. |
| Applications: | WB, IP, ChIP |
| Recommended Dilutions: | WB 1:500 - 1:2000 IP 1:50 - 1:200 ChIP 1:50 - 1:200 |
| Reactivity: | ALL |
| Positive Samples: | HeLa, MCF7, NIH/3T3, C6 |
| Immunogen: | A synthetic peptide corresponding to a sequence containing Tri-methylated K. |
| Purification Method: | Affinity purification |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Observed MW: | 15-74KDa |
 | Western blot analysis of extracts of various cell lines, using pan-Tri-Methyl K Motif antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
 | Immunoprecipitation analysis of 1. 5mg extracts of HeLa cells using 30ug pan-Tri-Methyl lysine antibody at a dilition of 1:1000. |
 | Chromatin immunoprecipitation analysis of extracts of HeLa cells, using pan-Tri-Methyl lysine antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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