Cell Biology Antibodies 18
Anti-Pan Mono-Methyl lysine Antibody (CAB18293)
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- SKU:
- CAB18293
- Product Type:
- Antibody
- Reactivity:
- Universal
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Pan Mono-Methyl lysine Antibody |
| Antibody SKU: | CAB18293 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | All |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence containing monomethylated K. |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | All |
| Positive Samples: | HeLa, MCF7, NIH/3T3, C6, Mouse liver, Rat liver |
| Immunogen: | A synthetic peptide corresponding to a sequence containing monomethylated K. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 17-90kDa |
 | Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
 | Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min. |
 | Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
 | Immunoprecipitation analysis of 1000ug extracts of HeLa cells using 20ug Pan Mono-Methyl lysine antibody at a dilition of 1:1000. |
 | Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Pan Mono-Methyl lysine antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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