Cell Biology Antibodies 16
Anti-Pan DiMethyl-lysine Antibody (CAB5870)
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- SKU:
- CAB5870
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Pan DiMethyl-lysine Antibody |
| Antibody SKU: | CAB5870 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence containing dimethylated K. |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Positive Samples: | HeLa acid extract, C6 acid extract, H3 protein |
| Immunogen: | A synthetic peptide corresponding to a sequence containing dimethylated K. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 15-60KDa |
| Synonyms: | |
| Background: |
 | Western blot analysis of extracts of various cell lines, using Pan DiMethyl-lysine antibody (CAB5870) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Dot-blot analysis of all sorts of methylation peptides using Pan DiMethyl-lysine antibody (CAB5870) at 1:1000 dilution. |
 | Immunofluorescence analysis of C6 cells using Pan DiMethyl-lysine antibody (CAB5870) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using Pan DiMethyl-lysine antibody (CAB5870) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine antibody (CAB5870) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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