Anti-PAH Antibody (CAB20949)

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SKU:
CAB20949
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-PAH Antibody (CAB20949)
Antibody SKU:CAB20949
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human PAH.
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, K-562, Mouse liver, Mouse kidney, Rat liver
Immunogen:Recombinant protein of Human PAH.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:
Calculated MW:51kDa
Observed MW:52KDa
Synonyms:PAH, PH, PKU, PKU1
Background:PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
Immunofluorescence analysis of rat liver cells using PAH Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of rat liver cells using PAH Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse liver cells using PAH Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of mouse liver cells using PAH Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PAH antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using PAH antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of HepG2 cells using PAH Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HepG2 cells using PAH Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PAH antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Western blot analysis of extracts of various cell lines, using PAH antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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