Anti-PAH Antibody (CAB20949)

Name: Anti-PAH Antibody (CAB20949)
SKU: CAB20949
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Metabolism Antibodies 1

system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक

OverviewProduct Name:Human PAH Recombinant Protein (His tag)Product Code:RPES5626Size:20µgSpecies:HumanExpressi

Antibody Name:	Anti-PAH Antibody (CAB20949)
Antibody SKU:	CAB20949
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunofluorescence
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of Human PAH.

Application:	Western blotting, Immunofluorescence
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, K-562, Mouse liver, Mouse kidney, Rat liver

Immunogen:	Recombinant protein of Human PAH.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Cellular Location:
Calculated MW:	51kDa
Observed MW:	52KDa

Synonyms:	PAH, PH, PKU, PKU1
Background:	PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

	Immunofluorescence analysis of rat liver cells using PAH Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse liver cells using PAH Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
	Western blot analysis of extracts of various cell lines, using PAH antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
	Immunofluorescence analysis of HepG2 cells using PAH Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
	Western blot analysis of extracts of various cell lines, using PAH antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.