Description
| Antibody Name: | Anti-PADI1 Antibody |
| Antibody SKU: | CAB10145 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PADI1 (NP_037490.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:1000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-431, SW480, U-937, HepG2, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PADI1 (NP_037490.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPK RVVQ LSLK MPTH AVCV VGVE AHVD IHSD VPKG ANSF RVSG SSGV EVFM VYNR TRVK EPIG KARW PLDT DADM VVSV GTAS KELK DFKV RVSY FGEQ EDQA LGRS VLYL TGVD ISLE VDTG RTGK VKRS QGDK KTWR WGPE GYGA ILLV NCDR DNHR SAEP DLTH SWLM SLAD LQDM SPML LSCN GPDK LFDS HKLV LNVP FSDS KRVR VFCA RGGN SLSD YKQV LGPQ CLSY EVER QPGE QEIK FYVE GLTF PDAD |
| Gene ID: | 29943 |
| Uniprot: | Q9ULC6 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 74kDa |
| Observed MW: | 65kDa |
| Synonyms: | PADI1, HPAD10, PAD1, PDI, PDI1 |
| Background: | This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes. |
| UniProt Protein Function: | Function: Catalyzes the deimination of arginine residues of proteins |
| UniProt Protein Details: | By similarity. Catalytic activity: Protein L-arginine + H2O = protein L-citrulline + NH3. Cofactor: Calcium By similarity. Subcellular location: Cytoplasm Ref.1. Tissue specificity: Epidermis, prostate, testis, placenta, spleen and thymus. Ref.1 Sequence similarities: Belongs to the protein arginine deiminase family. |
| NCBI Summary: | This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9ULC6 |
| NCBI GenInfo Identifier: | 122056684 |
| NCBI Gene ID: | 29943 |
| NCBI Accession: | NP_037490.2 |
| UniProt Secondary Accession: | Q9ULC6,Q70SX6, A1L4K6, |
| UniProt Related Accession: | Q9ULC6 |
| Molecular Weight: | 95 kDa |
| NCBI Full Name: | Homo sapiens peptidyl arginine deiminase, type I (PADI1), mRNA |
| NCBI Synonym Full Names: | peptidyl arginine deiminase, type I |
| NCBI Official Symbol: | PADI1 |
| NCBI Official Synonym Symbols: | PDI; PAD1; PDI1; HPAD10 |
| NCBI Protein Information: | protein-arginine deiminase type-1; hPAD-colony 10; peptidylarginine deiminase I; protein-arginine deiminase type I |
| UniProt Protein Name: | Protein-arginine deiminase type-1 |
| UniProt Synonym Protein Names: | Peptidylarginine deiminase I; Protein-arginine deiminase type I |
| Protein Family: | Proteasome |
| UniProt Gene Name: | PADI1 |
| UniProt Entry Name: | PADI1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PADI1 antibody (CAB10145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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