Antibody Name:	Polyclonal AntibodyPC4 Rabbit Polyclonal Antibody
Antibody SKU:	CAB5948
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC4 (NP_003810.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Jurkat, Mouse spleen, Mouse heart, Rat brain, Rat heart

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC4 (NP_003810.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VTEM NGRI VGSK PLYV ALAQ RKEE RKAH LTNQ YMQR VAGM RALP ANAI LNQF QPAA GGYF VPAV PQAQ GRPP YYTP NQLA QMRP NPRW QQGG RPQG FQGM P
Gene ID:	8761
Uniprot:	Q13310
Cellular Location:	Cytoplasm
Calculated MW:	69kDa/70kDa/72kDa
Observed MW:	79kDa

Synonyms:

PABPC4, APP-1, APP1, PABP4, iPABP

Background:

Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PABP 4: poly(A) binding protein 4 may be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated. Localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells.
UniProt Protein Details:	Protein type:RNA-binding; Translation Chromosomal Location of Human Ortholog: 1p34.2 Cellular Component: cytoplasm; nucleus; ribonucleoprotein complex; stress granule Molecular Function:poly(A) binding; poly(rC) binding; poly(U) binding; protein binding Biological Process: blood coagulation; RNA catabolic process; RNA processing; translation
NCBI Summary:	Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Code:	Q13310
NCBI GenInfo Identifier:	12229875
NCBI Gene ID:	8761
NCBI Accession:	Q13310.1
UniProt Secondary Accession:	Q13310,Q4VC03, Q6P0N3, B1ANQ8,
UniProt Related Accession:	Q13310
Molecular Weight:	72,391 Da
NCBI Full Name:	Polyadenylate-binding protein 4
NCBI Synonym Full Names:	poly(A) binding protein cytoplasmic 4
NCBI Official Symbol:	PABPC4
NCBI Official Synonym Symbols:	APP1; APP-1; PABP4; iPABP
NCBI Protein Information:	polyadenylate-binding protein 4
UniProt Protein Name:	Polyadenylate-binding protein 4
UniProt Synonym Protein Names:	Activated-platelet protein 1; APP-1; Inducible poly(A)-binding protein; iPABP
UniProt Gene Name:	PABPC4
UniProt Entry Name:	PABP4_HUMAN

	Western blot analysis of extracts of various cell lines, using PABPC4 antibody (CAB5948) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human esophagus using PABPC4 antibody (CAB5948) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PABPC4 antibody (CAB5948) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PABPC4 antibody (CAB5948) at dilution of 1:100. Blue: DAPI for nuclear staining.