Cell Death Antibodies 1

Anti-p70 S6 kinase Antibody (CAB2190)

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SKU:
CAB2190
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-p70 S6 kinase Antibody
Antibody SKU:CAB2190
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human P70 S6K (NP_003152.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human P70 S6K (NP_003152.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRRR RRRD GFYP APDF RDRE AEDM AGVF DIDL DQPE DAGS EDEL EEGG QLNE SMDH GGVG PYEL GMEH CEKF EISE TSVN RGPE KIRP ECFE LLRV LGKG GYGK VFQV RKVT GANT GKIF AMKV LKKA MIVR NAKD TAHT KAER NILE EVKH PFIV DLIY AFQT GGKL YLIL EYLS GGEL FMQL EREG IFME DTAC FYLA EISM ALGH LHQK GIIY RDLK PENI MLNH QGHV KLTD FGLC KESI HDGT VTHT FCGT IEYM APEI LMRS GHNR AVDW WSLG ALMY DMLT GAPP FTGE NRKK TIDK ILKC KLNL PPYL TQEA RDLL KKLL KRNA ASRL GAGP GDAG EVQA HPFF
Gene ID:6198
Uniprot:P23443
Cellular Location:Cell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome
Calculated MW:51kDa/52kDa/56kDa/59kDa
Observed MW:70KDa
Synonyms:PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha, P70 S6K, RPS6KB1, p70S6KA
Background:This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
UniProt Protein Function:p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.
UniProt Protein Details:

Protein type:EC 2.7.11.1; Translation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; AGC group; RSK family; p70 subfamily

Chromosomal Location of Human Ortholog: 17q23.1

NCBI Summary:This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
UniProt Code:P23443
NCBI GenInfo Identifier:54041234
NCBI Gene ID:6198
NCBI Accession:P23443.2
UniProt Secondary Accession:P23443,Q7Z721, B2R779, B4DLT4, B4DTG1, E7ESB8, F6UYM1
UniProt Related Accession:P23443
Molecular Weight:525
NCBI Full Name:Ribosomal protein S6 kinase beta-1
NCBI Synonym Full Names:ribosomal protein S6 kinase, 70kDa, polypeptide 1
NCBI Official Symbol:RPS6KB1  
NCBI Official Synonym Symbols:S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha  
NCBI Protein Information:ribosomal protein S6 kinase beta-1; p70 S6 kinase, alpha; ribosomal protein S6 kinase I; serine/threonine kinase 14 alpha; serine/threonine-protein kinase 14A
UniProt Protein Name:Ribosomal protein S6 kinase beta-1
UniProt Synonym Protein Names:70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
Protein Family:Ribosomal protein S6 kinase
UniProt Gene Name:RPS6KB1  
UniProt Entry Name:KS6B1_HUMAN
Anti-p70 S6 kinase Antibody (CAB2190)Western blot analysis of extracts of various cell lines, using p70 S6 kinase antibody (CAB2190) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-p70 S6 kinase Antibody (CAB2190)Immunohistochemistry of paraffin-embedded human breast cancer using p70 S6 kinase Rabbit pAb (CAB2190) at dilution of 1:100 (40x lens).
Anti-p70 S6 kinase Antibody (CAB2190)Immunofluorescence analysis of C6 cells using p70 S6 kinase Rabbit pAb (CAB2190) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-p70 S6 kinase Antibody (CAB2190)Immunofluorescence analysis of NIH-3T3 cells using p70 S6 kinase Rabbit pAb (CAB2190) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-p70 S6 kinase Antibody (CAB2190)Immunofluorescence analysis of U-2 OS cells using p70 S6 kinase Rabbit pAb (CAB2190) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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