Name: Anti-OXCT1 Antibody (CAB8139)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB8139
Availability: OutOfStock

Antibody Name:	[KO Validated] OXCT1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8139
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 261-520 of human OXCT1 (NP_000427.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, HeLa, Jurkat, 293T, Mouse kidney, Mouse heart, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 261-520 of human OXCT1 (NP_000427.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IPQI YVHR LIKG EKYE KRIE RLSI RKEG DGEA KSAK PGDD VRER IIKR AALE FEDG MYAN LGIG IPLL ASNF ISPN ITVH LQSE NGVL GLGP YPRQ HEAD ADLI NAGK ETVT ILPG ASFF SSDE SFAM IRGG HVDL TMLG AMQV SKYG DLAN WMIP GKMV KGMG GAMD LVSS AKTK VVVT MEHS AKGN AHKI MEKC TLPL TGKQ CVNR IITE KAVF DVDK KKGL TLIE LWEG LTVD DVQK STGC DFAV SPKL MPMQ QIAN
Gene ID:	5019
Uniprot:	P55809
Cellular Location:	Mitochondrion matrix
Calculated MW:	13kDa/56kDa
Observed MW:	56kDa

Synonyms:	OXCT1, OXCT, SCOT
Background:	This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.

UniProt Protein Function:	OXCT1: Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate. Defects in OXCT1 are a cause of succinyl-CoA-3-ketoacid- CoA transferase deficiency (SCOTD). A disorder of ketone body metabolism, characterized by episodic ketoacidosis. Patients are usually asymptomatic between episodes. Belongs to the 3-oxoacid CoA-transferase family.
UniProt Protein Details:	Protein type:Mitochondrial; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Transferase; Lipid Metabolism - synthesis and degradation of ketone bodies; EC 2.8.3.5 Chromosomal Location of Human Ortholog: 5p13.1 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:3-oxoacid CoA-transferase activity Biological Process: ketone body catabolic process; ketone body metabolic process Disease: Succinyl-coa:3-oxoacid Coa Transferase Deficiency
NCBI Summary:	This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008]
UniProt Code:	P55809
NCBI GenInfo Identifier:	2492998
NCBI Gene ID:	5019
NCBI Accession:	P55809.1
UniProt Secondary Accession:	P55809,B2R5V2, B7Z528,
UniProt Related Accession:	P55809
Molecular Weight:	13,358 Da
NCBI Full Name:	Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
NCBI Synonym Full Names:	3-oxoacid CoA-transferase 1
NCBI Official Symbol:	OXCT1
NCBI Official Synonym Symbols:	OXCT; SCOT
NCBI Protein Information:	succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
UniProt Protein Name:	Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
UniProt Synonym Protein Names:	3-oxoacid CoA-transferase 1; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; SCOT-s
Protein Family:	Succinyl-CoA:3-ketoacid coenzyme A transferase
UniProt Gene Name:	OXCT1
UniProt Entry Name:	SCOT1_HUMAN

	Western blot analysis of extracts of various cell lines, using OXCT1 antibody (CAB8139) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts from normal (control) and OXCT1 knockout (KO) HeLa cells, using OXCT1 antibody (CAB8139) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human esophagus using OXCT1 antibody (CAB8139) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using OXCT1 antibody (CAB8139) at dilution of 1:100 (40x lens).