Developmental Biology
Anti-OTP Antibody (CAB13188)
- SKU:
- CAB13188
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-OTP Antibody |
| Antibody SKU: | CAB13188 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human OTP (NP_115485.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse heart, Rat brain, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human OTP (NP_115485.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLSH ADLL DARL GMKD AAEL LGHR EAVK CRLG VGGS DPGG HPGD LAPN SDPV EGAT LLPG EDIT TVGS TPAS LAVS AKDP DKQP GPQG GPNP SQAG QQQG QQKQ KRHR TRFT PAQL NELE RSFA KTHY PD |
| Gene ID: | 23440 |
| Uniprot: | Q5XKR4 |
| Cellular Location: | Nucleus |
| Calculated MW: | 34kDa |
| Observed MW: | 34-36kDa |
| Synonyms: | OTP |
| Background: | This gene encodes a member of the homeodomain (HD) family. HD family proteins are helix-turn-helix transcription factors that play key roles in the specification of cell fates. This protein may function during brain development. |
| UniProt Protein Function: | OTP: Probably involved in the differentiation of hypothalamic neuroendocrine cells. Belongs to the paired homeobox family. Bicoid subfamily. |
| UniProt Protein Details: | Protein type:Transcription factor; Cell development/differentiation; DNA-binding Chromosomal Location of Human Ortholog: 5q13.3 |
| NCBI Summary: | This gene encodes a member of the homeodomain (HD) family. HD family proteins are helix-turn-helix transcription factors that play key roles in the specification of cell fates. This protein may function during brain development. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q5XKR4 |
| NCBI GenInfo Identifier: | 74762283 |
| NCBI Gene ID: | 23440 |
| NCBI Accession: | Q5XKR4.1 |
| UniProt Related Accession: | Q5XKR4 |
| Molecular Weight: | 34,159 Da |
| NCBI Full Name: | Homeobox protein orthopedia |
| NCBI Synonym Full Names: | orthopedia homeobox |
| NCBI Official Symbol: | OTP |
| NCBI Protein Information: | homeobox protein orthopedia |
| UniProt Protein Name: | Homeobox protein orthopedia |
| Protein Family: | Homeobox protein |
| UniProt Gene Name: | OTP |
| UniProt Entry Name: | OTP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using OTP antibody (CAB13188) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using OTP antibody (CAB13188) at dilution of 1:100 (40x lens). |
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