Antibody Name:	Anti-OSM Antibody
Antibody SKU:	CAB6163
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-252 of human OSM (NP_065391.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, NIH/3T3, Mouse uterus, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-252 of human OSM (NP_065391.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AAIG SCSK EYRV LLGQ LQKQ TDLM QDTS RLLD PYIR IQGL DVPK LREH CRER PGAF PSEE TLRG LGRR GFLQ TLNA TLGC VLHR LADL EQRL PKAQ DLER SGLN IEDL EKLQ MARP NILG LRNN IYCM AQLL DNSD TAEP TKAG RGAS QPPT PTPA SDAF QRKL EGCR FLHG YHRF MHSV GRVF SKWG ESPN RSRR HSPH QALR KGVR RTRP SRKG KRLM TRGQ LPR
Gene ID:	5008
Uniprot:	P13725
Cellular Location:	Secreted
Calculated MW:	28kDa
Observed MW:	28kDa

Synonyms:

OSM

Background:

This gene encodes a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells. This gene and the related gene, leukemia inhibitory factor, also present on chromosome 22, may have resulted from the duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

UniProt Protein Function:	OSM: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. Belongs to the LIF/OSM family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide; Cytokine Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: extracellular space Molecular Function:growth factor activity; oncostatin-M receptor binding; cytokine activity Biological Process: negative regulation of hormone secretion; multicellular organismal development; tyrosine phosphorylation of Stat3 protein; positive regulation of peptidyl-serine phosphorylation; tyrosine phosphorylation of Stat5 protein; peripheral nervous system development; cell proliferation; negative regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; behavioral response to pain; positive regulation of MAPKKK cascade; positive regulation of acute inflammatory response; response to heat; positive regulation of cell division; regulation of growth; positive regulation of cell proliferation; negative regulation of meiosis; tyrosine phosphorylation of Stat1 protein; positive regulation of transcription from RNA polymerase II promoter; immune response
NCBI Summary:	Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. [provided by RefSeq, Jul 2008]
UniProt Code:	P13725
NCBI GenInfo Identifier:	129168
NCBI Gene ID:	5008
NCBI Accession:	P13725.2
UniProt Secondary Accession:	P13725,Q6FHP8, Q9UCP6,
UniProt Related Accession:	P13725
Molecular Weight:	28,484 Da
NCBI Full Name:	Oncostatin-M
NCBI Synonym Full Names:	oncostatin M
NCBI Official Symbol:	OSM
NCBI Protein Information:	oncostatin-M
UniProt Protein Name:	Oncostatin-M
Protein Family:	Oncostatin
UniProt Gene Name:	OSM
UniProt Entry Name:	ONCM_HUMAN

	Western blot analysis of extracts of various cell lines, using OSM Antibody (CAB6163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human prostate using OSM antibody (CAB6163) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using OSM antibody (CAB6163) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L-929 cells using OSM antibody (CAB6163) at dilution of 1:100. Blue: DAPI for nuclear staining.