Antibody Name:	Anti-OPN4 Antibody
Antibody SKU:	CAB16289
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 365-489 of human OPN4 (NP_001025186.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	mouse eye

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 365-489 of human OPN4 (NP_001025186.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HPKY RVAI AQHL PCLG VLLG VSRR HSRP YPSY RSTH RSTL TSHT SNLS WISI RRRQ ESLG SESE VGWT HMEA AAVW GAAQ QANG RSLY GQGL EDLE AKAP PRPQ GHEA ETPG KTKG LIPS QDPR M
Gene ID:	94233
Uniprot:	Q9UHM6
Cellular Location:
Calculated MW:	52kDa/53kDa
Observed MW:

Synonyms:

OPN4, MOP, opsin 4

Background:

Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal.
NCBI Summary:	Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UHM6
NCBI GenInfo Identifier:	71999143
NCBI Gene ID:	94233
NCBI Accession:	NP_001025186.1
UniProt Secondary Accession:	Q9UHM6,Q14D01, Q2PP22, Q8NGQ9, B7ZLB3,
UniProt Related Accession:	Q9UHM6
Molecular Weight:	55 kDa
NCBI Full Name:	melanopsin isoform 2
NCBI Synonym Full Names:	opsin 4
NCBI Official Symbol:	OPN4
NCBI Official Synonym Symbols:	MOP
NCBI Protein Information:	melanopsin
UniProt Protein Name:	Melanopsin
UniProt Synonym Protein Names:	Opsin-4
Protein Family:	Melanopsin
UniProt Gene Name:	OPN4

	Western blot analysis of extracts of mouse eye, using OPN4 antibody (CAB16289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat retina using OPN4 antibody (CAB16289) at dilution of 1:200 (40x lens).