Antibody Name:	Anti-OLIG2 Antibody
Antibody SKU:	CAB12814
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human OLIG2 (NP_005797.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human OLIG2 (NP_005797.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDSD ASLV SSRP SSPE PDDL FLPA RSKG SSGS AFTG GTVS SSTP SDCP PELS AELR GAMG SAGA HPGD KLGG SGFK SSSS
Gene ID:	10215
Uniprot:	Q13516
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	32kDa
Observed MW:	32kDa

Synonyms:

OLIG2, BHLHB1, OLIGO2, PRKCBP2, RACK17, bHLHe19

Background:

This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome.

UniProt Protein Function:	OLIG2: Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
UniProt Protein Details:	Protein type:Transcription factor; Oncoprotein Chromosomal Location of Human Ortholog: 21q22.11
NCBI Summary:	This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13516
NCBI GenInfo Identifier:	22261817
NCBI Gene ID:	10215
NCBI Accession:	Q13516.2
UniProt Secondary Accession:	Q13516,Q05BP9, Q49AL3, Q86X04, Q9NZ14, B3KRF3,
UniProt Related Accession:	Q13516
Molecular Weight:	32,385 Da
NCBI Full Name:	Oligodendrocyte transcription factor 2
NCBI Synonym Full Names:	oligodendrocyte lineage transcription factor 2
NCBI Official Symbol:	OLIG2
NCBI Official Synonym Symbols:	BHLHB1; OLIGO2; RACK17; PRKCBP2; bHLHe19
NCBI Protein Information:	oligodendrocyte transcription factor 2
UniProt Protein Name:	Oligodendrocyte transcription factor 2
UniProt Synonym Protein Names:	Class B basic helix-loop-helix protein 1; bHLHb1; Class E basic helix-loop-helix protein 19; bHLHe19; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17
Protein Family:	Oligodendrocyte transcription factor
UniProt Gene Name:	OLIG2
UniProt Entry Name:	OLIG2_HUMAN

	Western blot analysis of extracts of 293T cells, using OLIG2 antibody (CAB12814) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using OLIG2 antibody (CAB12814) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using OLIG2 antibody (CAB12814) at dilution of 1:200 (40x lens).