Epigenetics & Nuclear Signaling Antibodies 3

Anti-OGG1 Antibody (CAB2268)

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SKU:
CAB2268
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-OGG1 Antibody
Antibody SKU:CAB2268
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide of human OGG1
Application:WB
Recommended Dilution:WB 1:200 - 1:1000
Reactivity:Human, Mouse
Positive Samples:HepG2
Immunogen:A synthetic peptide of human OGG1
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4968
Uniprot:O15527
Cellular Location:Mitochondrion, Nucleus, Nucleus matrix, Nucleus speckle, nucleoplasm
Calculated MW:22kDa/36kDa/38kDa/39kDa/40kDa/45kDa/47kDa
Observed MW:36kDa
Synonyms:OGG1, HMMH, HOGG1, MUTM, OGH1
Background:This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.
UniProt Protein Function:OGG1: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3' to the lesion. Defects in OGG1 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Belongs to the type-1 OGG1 family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 4.2.99.18; Lyase; DNA repair, damage; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 3p26.2

Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; nuclear speck

Molecular Function:protein binding; microtubule binding; endonuclease activity; DNA N-glycosylase activity; oxidized purine base lesion DNA N-glycosylase activity; damaged DNA binding; 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity

Biological Process: response to drug; depurination; DNA repair; DNA catabolic process, endonucleolytic; response to estradiol stimulus; response to radiation; response to ethanol; base-excision repair, AP site formation; regulation of transcription, DNA-dependent; nucleotide-excision repair; base-excision repair; response to folic acid; regulation of protein import into nucleus, translocation; response to oxidative stress; acute inflammatory response; negative regulation of apoptosis; aging

Disease: Renal Cell Carcinoma, Nonpapillary

NCBI Summary:This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]
UniProt Code:O15527
NCBI GenInfo Identifier:12643548
NCBI Gene ID:4968
NCBI Accession:O15527.2
UniProt Secondary Accession:O15527,O00390, O00670, O00705, O14876, O95488, P78554 Q9BW42, Q9UIK0, Q9UIK1, Q9UIK2, A8K1E3,
UniProt Related Accession:O15527
Molecular Weight:345
NCBI Full Name:N-glycosylase/DNA lyase
NCBI Synonym Full Names:8-oxoguanine DNA glycosylase
NCBI Official Symbol:OGG1  
NCBI Official Synonym Symbols:HMMH; MUTM; OGH1; HOGG1  
NCBI Protein Information:N-glycosylase/DNA lyase; AP lyase; OGG1 type 1f; 8-hydroxyguanine DNA glycosylase; DNA-apurinic or apyrimidinic site lyase
UniProt Protein Name:N-glycosylase/DNA lyase
Protein Family:N-glycosylase/DNA lyase
UniProt Gene Name:OGG1  
UniProt Entry Name:OGG1_HUMAN
Anti-OGG1 Antibody (CAB2268)Western blot analysis of extracts of HepG2 cells, using OGG1 antibody (CAB2268) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
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