Epigenetics & Nuclear Signaling Antibodies 4

Anti-OCT4 Antibody (CAB7920)

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SKU:
CAB7920
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-OCT4 Antibody
Antibody SKU:CAB7920
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human OCT4
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:NIH/3T3, Mouse testis, Mouse brain, Mouse ovary, Rat testis
Immunogen:A synthetic peptide of human OCT4
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5460
Uniprot:Q01860
Cellular Location:Cytoplasm, Nucleus
Calculated MW:30kDa/38kDa
Observed MW:50kDa
Synonyms:POU5F1, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4
Background:This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
UniProt Protein Function:Oct4: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function:protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; blastocyst development; anatomical structure morphogenesis; regulation of asymmetric cell division; regulation of transcription, DNA-dependent; regulation of gene expression; somatic stem cell maintenance; endodermal cell fate specification; response to wounding; positive regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]
UniProt Code:Q01860
NCBI GenInfo Identifier:42560248
NCBI Gene ID:5460
NCBI Accession:NP_002692
UniProt Secondary Accession:Q01860,P31359, Q15167, Q15168, Q16422, Q5STF3, Q5STF4 A6NCS1, A6NLL8, D2IYK4,
UniProt Related Accession:Q01860
Molecular Weight:30,085 Da
NCBI Full Name:POU domain, class 5, transcription factor 1 isoform 1
NCBI Synonym Full Names:POU class 5 homeobox 1
NCBI Official Symbol:POU5F1  
NCBI Official Synonym Symbols:OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4  
NCBI Protein Information:POU domain, class 5, transcription factor 1; octamer-binding protein 3; octamer-binding protein 4; POU domain transcription factor OCT4; octamer-binding transcription factor 3; POU-type homeodomain-containing DNA-binding protein
UniProt Protein Name:POU domain, class 5, transcription factor 1
UniProt Synonym Protein Names:Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3
UniProt Gene Name:POU5F1  
UniProt Entry Name:PO5F1_HUMAN
Anti-OCT4 Antibody (CAB7920)Western blot analysis of extracts of various cell lines, using OCT4 antibody (CAB7920) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-OCT4 Antibody (CAB7920)Immunohistochemistry of paraffin-embedded human brain cancer using OCT4 antibody (CAB7920) at dilution of 1:100 (40x lens).
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