Cell Biology Antibodies 2

Anti-NUDT5 Antibody (CAB10493)

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SKU:
CAB10493
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-NUDT5 Antibody
Antibody SKU:CAB10493
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-219 of human NUDT5 (NP_054861.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, LO2, DU145, HT-1080, Mouse liver, Mouse kidney, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-219 of human NUDT5 (NP_054861.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MESQ EPTE SSQN GKQY IISE ELIS EGKW VKLE KTTY MDPT GKTR TWES VKRT TRKE QTAD GVAV IPVL QRTL HYEC IVLV KQFR PPMG GYCI EFPA GLID DGET PEAA ALRE LEEE TGYK GDIA ECSP AVCM DPGL SNCT IHIV TVTI NGDD AENA RPKP KPGD GEFV EVIS LPKN DLLQ RLDA LVAE EHLT VDAR VYSY ALAL KHAN AKPF EVPF LKF
Gene ID:11164
Uniprot:Q9UKK9
Cellular Location:
Calculated MW:24kDa
Observed MW:36kDa
Synonyms:NUDT5, YSA1, YSA1H, YSAH1, hNUDT5
Background:This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors.
UniProt Protein Function:NUDT5: Hydrolyzes with similar activities ADP-ribose ADP- mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Can also hydrolyze other nucleotide sugars with low activity. Belongs to the Nudix hydrolase family.
UniProt Protein Details:

Protein type:EC 3.6.1.13; EC 3.6.1.58; Nucleotide Metabolism - purine; Phosphatase

Chromosomal Location of Human Ortholog: 10p14

Cellular Component: cytosol; nucleus

Molecular Function:ADP-ribose diphosphatase activity; ADP-sugar diphosphatase activity; m7G(5')pppN diphosphatase activity; magnesium ion binding; nucleotidyltransferase activity; protein binding; protein homodimerization activity; snoRNA binding

Biological Process: chromatin remodeling; D-ribose catabolic process; ribonucleoside diphosphate catabolic process

NCBI Summary:This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors. [provided by RefSeq, Aug 2013]
UniProt Code:Q9UKK9
NCBI GenInfo Identifier:20455192
NCBI Gene ID:11164
NCBI Accession:Q9UKK9.1
UniProt Secondary Accession:Q9UKK9,Q6IAG0, Q9UH49, A8K516,
UniProt Related Accession:Q9UKK9
Molecular Weight:24kDa
NCBI Full Name:ADP-sugar pyrophosphatase
NCBI Synonym Full Names:nudix hydrolase 5
NCBI Official Symbol:NUDT5  
NCBI Official Synonym Symbols:YSA1; YSA1H; YSAH1; hNUDT5  
NCBI Protein Information:ADP-sugar pyrophosphatase
UniProt Protein Name:ADP-sugar pyrophosphatase
UniProt Synonym Protein Names:8-oxo-dGDP phosphatase (EC:3.6.1.58
Protein Family:Nudix hydrolase
UniProt Gene Name:NUDT5  
Anti-NUDT5 Antibody (CAB10493)Western blot analysis of extracts of various cell lines, using NUDT5 antibody (CAB10493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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