Anti-NUCKS1 Antibody (CAB21091)
- SKU:
- CAB21091
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- NHP - Monkey
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-NUCKS1 Antibody (CAB21091) |
| Antibody SKU: | CAB21091 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human NUCKS1 (NP_073568.2). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, HepG2, HeLa, Rat brain, Mouse lung |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human NUCKS1 (NP_073568.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ASKQ REML MEDV GSEE EQEE EDEA PFQE KDSG SDED FLME DDDD SDYG SSKK KNKK MVKK SKPE RKEK KMPK PRLK ATVT PSPV KGKG KVGR PTAS KASK E |
| Cellular Location: | |
| Calculated MW: | 27kDa |
| Observed MW: | 45KDa |
| Synonyms: | JC7, NUCKS |
| Background: | This gene encodes a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated in vivo by Cdk1 during mitosis of the cell cycle. |
 | Western blot analysis of extracts of Mouse lung, using NUCKS1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
 | Western blot analysis of extracts of various cell lines, using NUCKS1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
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