Cell Biology Antibodies 6

Anti-NTAN1 Antibody (CAB15562)

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SKU:
CAB15562
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NTAN1 Antibody
Antibody SKU:CAB15562
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human NTAN1 (NP_775745.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Raji, HepG2, MCF-7, Mouse spleen, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human NTAN1 (NP_775745.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPLL VEGR RVRL PQSA GDLV RAHP PLEE RARL LRGQ SVQQ VGPQ GLLY VQQR ELAV TSPK DGSI SILG SDDA TTCH IVVL RHTG NGAT CLTH CDGT DTKA EVPL IMNS IKSF SDHA QCGR LEVH LVGG FSDD RQLS QKLT HQLL SEFD RQED DIHL VTLC VTEL NDRE ENEN HFPV IYGI AVNI KTAE IYRA SFQD RGPE EQLR AART LAGG PMIS IYDA ETEQ LRIG PYSW TPFP HVDF WLHQ DDKQ ILEN LSTS PLAE PPHF VEHI RSTL MFLK KHPS PAHT LFSG NKAL LYKK NEDG LWEK ISSP GS
Gene ID:123803
Uniprot:Q96AB6
Cellular Location:Cytoplasm
Calculated MW:34kDa
Observed MW:35kDa
Synonyms:NTAN1, PNAA, PNAD
Background:The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
UniProt Protein Function:NTAN1: Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N- terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position.
UniProt Protein Details:

Protein type:Hydrolase; EC 3.5.1.-

Chromosomal Location of Human Ortholog: 16p13.11

NCBI Summary:The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]
UniProt Code:Q96AB6
NCBI GenInfo Identifier:37082118
NCBI Gene ID:123803
NCBI Accession:Q96AB6.3
UniProt Secondary Accession:Q96AB6,Q7Z4Z0,
UniProt Related Accession:Q96AB6
Molecular Weight:34,677 Da
NCBI Full Name:Protein N-terminal asparagine amidohydrolase
NCBI Synonym Full Names:N-terminal asparagine amidase
NCBI Official Symbol:NTAN1  
NCBI Official Synonym Symbols:PNAA; PNAD  
NCBI Protein Information:protein N-terminal asparagine amidohydrolase
UniProt Protein Name:Protein N-terminal asparagine amidohydrolase
UniProt Synonym Protein Names:Protein NH2-terminal asparagine amidohydrolase; PNAA; Protein NH2-terminal asparagine deamidase; PNAD; Protein N-terminal Asn amidase; Protein N-terminal asparagine amidase; Protein NTN-amidase
Protein Family:Protein N-terminal asparagine amidohydrolase
UniProt Gene Name:NTAN1  
UniProt Entry Name:NTAN1_HUMAN
Anti-NTAN1 Antibody (CAB15562)Western blot analysis of extracts of various cell lines, using NTAN1 antibody (CAB15562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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