Autophagy Antibodies

Anti-NRBF2 Antibody (CAB13422)

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SKU:
CAB13422
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy

Description

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Antibody Name:Anti-NRBF2 Antibody
Antibody SKU:CAB13422
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 10-220 of human NRBF2 (NP_110386.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, 293T, HeLa, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 10-220 of human NRBF2 (NP_110386.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LAHQ QSRR ADRL LAAG KYEE AISC HKKA AAYL SEAM KLTQ SEQA HLSL ELQR DSHM KQLL LIQE RWKR AQRE ERLK AQQN TDKD AAAH LQTS HKPS AEDA EGQS PLSQ KYSP STEK CLPE IQGI FDRD PDTL LYLL QQKS EPAE PCIG SKAP KDDK TIIE EQAT KIAD LKRH VEFL VAEN ERLR KENK QLKA EKAR LLKG PIEK ELDV DAD
Gene ID:29982
Uniprot:Q96F24
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Nucleus, autophagosome
Calculated MW:26kDa/31kDa/32kDa
Observed MW:38kDa
Synonyms:NRBF2, COPR, COPR1, COPR2, NRBF-2
Background:
UniProt Protein Function:NRBF2: May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro). Interacts with PPARA, PPARD and PPARG. Interacts with RARA, RARG and RXRA in the presence of bound ligand. Detected in keratinocytes, liver and placenta. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; Autophagy; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 10q21.3

Cellular Component: nucleoplasm; cytoplasm

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; gene expression

UniProt Code:Q96F24
NCBI GenInfo Identifier:74731648
NCBI Gene ID:29982
NCBI Accession:Q96F24.1
UniProt Secondary Accession:Q96F24,Q86UR2, Q96NP6, Q9H0S9, Q9H2I2, A6PW36, B4DWS0
UniProt Related Accession:Q96F24
Molecular Weight:287
NCBI Full Name:Nuclear receptor-binding factor 2
NCBI Synonym Full Names:nuclear receptor binding factor 2
NCBI Official Symbol:NRBF2  
NCBI Official Synonym Symbols:COPR1; COPR2; NRBF-2  
NCBI Protein Information:nuclear receptor-binding factor 2; comodulator of PPAR and RXR 1; comodulator of PPAR and RXR 2; nuclear receptor binding factor-2
UniProt Protein Name:Nuclear receptor-binding factor 2
UniProt Synonym Protein Names:Comodulator of PPAR and RXR
Protein Family:Nuclear receptor-binding factor
UniProt Gene Name:NRBF2  
UniProt Entry Name:NRBF2_HUMAN
Anti-NRBF2 Antibody (CAB13422)Western blot analysis of extracts of various cell lines, using NRBF2 antibody (CAB13422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-NRBF2 Antibody (CAB13422)Immunohistochemistry of paraffin-embedded human esophageal using NRBF2 Rabbit pAb (CAB13422) at dilution of 1:100 (40x lens).
Anti-NRBF2 Antibody (CAB13422)Immunofluorescence analysis of C6 cells using NRBF2 Rabbit pAb (CAB13422) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-NRBF2 Antibody (CAB13422)Immunofluorescence analysis of HeLa cells using NRBF2 Rabbit pAb (CAB13422) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-NRBF2 Antibody (CAB13422)Immunofluorescence analysis of NIH-3T3 cells using NRBF2 Rabbit pAb (CAB13422) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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