The Steroidogenic factor-1 (SF-1/NR5A1) Antibody (CAB1657) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications.NR5A1, also known as steroidogenic factor 1 (SF-1), plays a crucial role in regulating genes involved in steroidogenesis and sexual development. Dysregulation of NR5A1 has been implicated in conditions such as adrenal insufficiency, gonadal dysgenesis, and reproductive disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Steroidogenic factor-1 (SF-1/NR5A1) Antibody
SKU:
CAB1657
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect.
Purification Method
Affinity purification
Gene ID
2516
RRID
AB_2763714
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse testis, using Steroidogenic factor-1 (SF-1/NR5A1) Rabbit pAb (CAB1657) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of various lysates using Steroidogenic factor-1 (SF-1/NR5A1) Rabbit pAb (CAB1657) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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