Name: Anti-NR2F2 Antibody (CAB10251)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB10251
Availability: OutOfStock

Antibody Name:	Anti-NR2F2 Antibody
Antibody SKU:	CAB10251
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NR2F2 (NP_066285.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, 293T

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NR2F2 (NP_066285.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAMV VSTW RDPQ DEVP GSQG SQAS QAPP VPGP PPGA PHTP QTPG QGGP ASTP AQTA AGGQ GGPG GPGS DKQQ QQQH IECV VCGD KSSG KHYG QFTC EGCK
Gene ID:	7026
Uniprot:	P24468
Cellular Location:	Nucleus
Calculated MW:	29kDa/31kDa/45kDa
Observed MW:	45kDa

Synonyms:	NR2F2, ARP1, CHTD4, COUPTFB, COUPTFII, NF-E3, NR2F1, SVP40, TFCOUP2
Background:	This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	NR2F2: Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. Interacts with SQSTM1. Binds DNA as a dimer; homodimer or heterodimer with NR2F6. Interacts with NCOA1, NCOA2, NCOA3 and PPARGC1A. Interacts with ZFPM2. Ubiquitous. Belongs to the nuclear hormone receptor family. NR2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor Chromosomal Location of Human Ortholog: 15q26.2 Cellular Component: nucleus Molecular Function:ligand-dependent nuclear receptor activity; protein binding; protein homodimerization activity; retinoic acid binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity Biological Process: lipid metabolic process; negative regulation of cyclin-dependent protein kinase activity; negative regulation of endothelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; signal transduction Disease: Congenital Heart Defects, Multiple Types, 4
NCBI Summary:	This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
UniProt Code:	P24468
NCBI GenInfo Identifier:	114203
NCBI Gene ID:	7026
NCBI Accession:	P24468.1
UniProt Secondary Accession:	P24468,Q03754, Q3KQR7, B4DQJ2, B6ZGU1,
UniProt Related Accession:	P24468
Molecular Weight:	46kDa
NCBI Full Name:	COUP transcription factor 2
NCBI Synonym Full Names:	nuclear receptor subfamily 2 group F member 2
NCBI Official Symbol:	NR2F2
NCBI Official Synonym Symbols:	ARP1; CHTD4; NF-E3; NR2F1; SVP40; COUPTFB; TFCOUP2; COUPTFII
NCBI Protein Information:	COUP transcription factor 2
UniProt Protein Name:	COUP transcription factor 2
UniProt Synonym Protein Names:	Apolipoprotein A-I regulatory protein 1; ARP-1; COUP transcription factor II; COUP-TF II; Nuclear receptor subfamily 2 group F member 2
Protein Family:	COUP transcription factor
UniProt Gene Name:	NR2F2

	Western blot analysis of extracts of various cell lines, using NR2F2 antibody (CAB10251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using NR2F2 Rabbit pAb (CAB10251) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using NR2F2 Rabbit pAb (CAB10251) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using NR2F2 Rabbit pAb (CAB10251) at dilution of 1:100. Blue: DAPI for nuclear staining.