KO Validated Antibodies 1

Anti-NOX4 Antibody (CAB11274)[KO Validated]

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SKU:
CAB11274
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NOX4 Antibody [KO Validated]
Antibody SKU:CAB11274
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 328-578 of human NOX4 (NP_058627.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 328-578 of human NOX4 (NP_058627.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KENF KARP GQYI TLHC PSVS ALEN HPFT LTMC PTET KATF GVHL KIVG DWTE RFRD LLLP PSSQ DSEI LPFI QSRN YPKL YIDG PFGS PFEE SLNY EVSL CVAG GIGV TPFA SILN TLLD DWKP YKLR RLYF IWVC RDIQ SFRW FADL LCML HNKF WQEN RPDY VNIQ LYLS QTDG IQKI IGEK YHAL NSRL FIGR PRWK LLFD EIAK YNRG KTVG VFCC GPNS LSKT LHKL SNQN NSYG TRFE YNKE SFS
Gene ID:50507
Uniprot:Q9NPH5
Cellular Location:Cell junction, Cell membrane, Endoplasmic reticulum membrane, Multi-pass membrane protein, Nucleus, focal adhesion, nucleolus
Calculated MW:6kDa/25-31kDa/58-66kDa
Observed MW:65KDa
Synonyms:NOX4, KOX, KOX-1, RENOX
Background:This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:NOX4: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; EC 1.6.3.-; Membrane protein, multi-pass; Cell cycle regulation; Nucleolus; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q14.2-q21

Cellular Component: endoplasmic reticulum membrane; focal adhesion; mitochondrion; perinuclear region of cytoplasm; apical plasma membrane; nucleolus; integral to membrane; stress fiber; NADPH oxidase complex

Molecular Function:oxygen sensor activity; electron carrier activity; FAD binding; NAD(P)H oxidase activity; superoxide-generating NADPH oxidase activity; nucleotide binding; heme binding

Biological Process: positive regulation of apoptosis; cell morphogenesis; cell aging; homocysteine metabolic process; positive regulation of smooth muscle cell migration; cardiac muscle cell differentiation; positive regulation of MAP kinase activity; negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of stress fiber formation; response to hypoxia; inflammatory response; superoxide release; bone resorption

NCBI Summary:This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]
UniProt Code:Q9NPH5
NCBI GenInfo Identifier:212276447
NCBI Gene ID:50507
NCBI Accession:Q9NPH5.2
UniProt Secondary Accession:Q9NPH5,Q5K3R4, Q5K3R5, Q5K3R6, Q5K3R8, Q7Z7G3, Q86V92 A8K715, B7Z520, E7EMD7,
UniProt Related Accession:Q9NPH5
Molecular Weight:Calculated MW: 6kDa/25-31kDa/58-66kDaObserved MW: 67 kDa
NCBI Full Name:NADPH oxidase 4
NCBI Synonym Full Names:NADPH oxidase 4
NCBI Official Symbol:NOX4  
NCBI Official Synonym Symbols:KOX; KOX-1; RENOX  
NCBI Protein Information:NADPH oxidase 4; kidney oxidase-1; renal NAD(P)H-oxidase; kidney superoxide-producing NADPH oxidase
UniProt Protein Name:NADPH oxidase 4
UniProt Synonym Protein Names:Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase
Protein Family:NADPH oxidase
UniProt Gene Name:NOX4  
UniProt Entry Name:NOX4_HUMAN
Anti-NOX4 Antibody [KO Validated] (CAB11274)Western blot analysis of extracts of 293T cells, using NOX4 antibody (CAB11274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NOX4 Antibody [KO Validated] (CAB11274)Western blot analysis of extracts from normal (control) and NOX4 Rabbit pAb knockout (KO) HeLa cells, using NOX4 Rabbit pAb antibody (CAB11274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-NOX4 Antibody [KO Validated] (CAB11274)Immunohistochemistry of paraffin-embedded rat lung using NOX4 Rabbit pAb (CAB11274) at dilution of 1:100 (40x lens).
Anti-NOX4 Antibody [KO Validated] (CAB11274)Immunohistochemistry of paraffin-embedded rat kidney using NOX4 Rabbit pAb (CAB11274) at dilution of 1:100 (40x lens).
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