Developmental Biology
Anti-NOTCH3 Antibody (CAB0929)
- SKU:
- CAB0929
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-NOTCH3 Antibody |
| Antibody SKU: | CAB0929 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 751-850 of human NOTCH3 (NP_000426.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 751-850 of human NOTCH3 (NP_000426.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SDGM GFHC TCPP GVQG RQCE LLSP CTPN PCEH GGRC ESAP GQLP VCSC PQGW QGPR CQQD VDEC AGPA PCGP HGIC TNLA GSFS CTCH GGYT GPSC DQDI |
| Gene ID: | 4854 |
| Uniprot: | Q9UM47 |
| Cellular Location: | Cell membrane, Nucleus, Single-pass type I membrane protein |
| Calculated MW: | 243kDa |
| Observed MW: | Refer to figures |
| Synonyms: | NOTCH3, CADASIL, CADASIL1, CASIL, IMF2, LMNS, notch 3 |
| Background: | This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). |
| UniProt Protein Function: | NOTCH3: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3. Interacts with PSMA1. Interacts with HIF1AN. Ubiquitously expressed in fetal and adult tissues. Belongs to the NOTCH family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 19p13.2-p13.1 Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cytoplasm; plasma membrane; extracellular region; integral to membrane; cytosol; receptor complex; actin cytoskeleton Molecular Function:protein binding; enzyme binding; calcium ion binding Biological Process: transcription initiation from RNA polymerase II promoter; negative regulation of neuron differentiation; Notch signaling pathway; regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; forebrain development; neuron fate commitment; Notch receptor processing; gene expression Disease: Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy; Lateral Meningocele Syndrome; Myofibromatosis, Infantile, 2 |
| NCBI Summary: | This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UM47 |
| NCBI GenInfo Identifier: | 322510053 |
| NCBI Gene ID: | 4854 |
| NCBI Accession: | Q9UM47.2 |
| UniProt Secondary Accession: | Q9UM47,Q9UEB3, Q9UPL3, Q9Y6L8, |
| UniProt Related Accession: | Q9UM47 |
| Molecular Weight: | 2321 |
| NCBI Full Name: | Neurogenic locus notch homolog protein 3 |
| NCBI Synonym Full Names: | notch 3 |
| NCBI Official Symbol: | NOTCH3 |
| NCBI Official Synonym Symbols: | IMF2; CASIL; CADASIL |
| NCBI Protein Information: | neurogenic locus notch homolog protein 3; Notch homolog 3 |
| UniProt Protein Name: | Neurogenic locus notch homolog protein 3 |
| Protein Family: | Neurogenic locus notch homolog protein |
| UniProt Gene Name: | NOTCH3 |
| UniProt Entry Name: | NOTC3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NOTCH3 antibody (CAB0929) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 15s. |
 | Immunofluorescence analysis of L929 cells using NOTCH3 Rabbit pAb (CAB0929) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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