Cell Biology Antibodies 10
Anti-NMT2 Antibody (CAB7042)
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- SKU:
- CAB7042
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NMT2 Antibody |
| Antibody SKU: | CAB7042 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human NMT2 (NP_004799.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Raji, 293T, SW480, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human NMT2 (NP_004799.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAED SESA ASQQ SLEL DDQD TCGI DGDN EEET EHAK GSPG GYLG AKKK KKKQ KRKK EKPN SGGT KSDS ASDS QEIK IQQP SKNP SVPM QKLQ DIQR AMEL LSAC QGPA RNID EAAK HRYQ |
| Gene ID: | 9397 |
| Uniprot: | O60551 |
| Cellular Location: | Cytoplasm, Membrane, Peripheral membrane protein |
| Calculated MW: | 56kDa |
| Observed MW: | 57kDa |
| Synonyms: | NMT2 |
| Background: | This gene encodes one of two N-myristoyltransferase proteins. N-terminal myristoylation is a lipid modification that is involved in regulating the function and localization of signaling proteins. The encoded protein catalyzes the addition of a myristoyl group to the N-terminal glycine residue of many signaling proteins, including the human immunodeficiency virus type 1 (HIV-1) proteins, Gag and Nef. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NMT2: Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Belongs to the NMT family. |
| UniProt Protein Details: | Protein type:EC 2.3.1.97; Transferase Chromosomal Location of Human Ortholog: 10p13 Cellular Component: cytoplasm; cytosol; extrinsic to membrane; Golgi apparatus; plasma membrane Molecular Function:glycylpeptide N-tetradecanoyltransferase activity Biological Process: N-terminal peptidyl-glycine N-myristoylation; regulation of rhodopsin mediated signaling |
| NCBI Summary: | This gene encodes one of two N-myristoyltransferase proteins. N-terminal myristoylation is a lipid modification that is involved in regulating the function and localization of signaling proteins. The encoded protein catalyzes the addition of a myristoyl group to the N-terminal glycine residue of many signaling proteins, including the human immunodeficiency virus type 1 (HIV-1) proteins, Gag and Nef. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
| UniProt Code: | O60551 |
| NCBI GenInfo Identifier: | 6225770 |
| NCBI Gene ID: | 9397 |
| NCBI Accession: | O60551.1 |
| UniProt Secondary Accession: | O60551,Q53Y38, Q5VUC8, Q9BRB4, B0YJ49, |
| UniProt Related Accession: | O60551 |
| Molecular Weight: | 57kDa |
| NCBI Full Name: | Glycylpeptide N-tetradecanoyltransferase 2 |
| NCBI Synonym Full Names: | N-myristoyltransferase 2 |
| NCBI Official Symbol: | NMT2 |
| NCBI Protein Information: | glycylpeptide N-tetradecanoyltransferase 2 |
| UniProt Protein Name: | Glycylpeptide N-tetradecanoyltransferase 2 |
| UniProt Synonym Protein Names: | Myristoyl-CoA:protein N-myristoyltransferase 2; NMT 2; Peptide N-myristoyltransferase 2; Type II N-myristoyltransferase |
| Protein Family: | Glycylpeptide N-tetradecanoyltransferase |
| UniProt Gene Name: | NMT2 |
 | Western blot analysis of extracts of various cell lines, using NMT2 antibody (CAB7042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using NMT2 antibody (CAB7042) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A-549 cells using NMT2 antibody (CAB7042). Blue: DAPI for nuclear staining. |
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