Metabolism Antibodies 1

Anti-NMNAT2 Antibody (CAB13833)

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SKU:
CAB13833
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-NMNAT2 Antibody
Antibody SKU:CAB13833
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human NMNAT2 (NP_733820.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, NCI-H460, BxPC-3, Mouse pancreas, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human NMNAT2 (NP_733820.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEIQ ELEE IQAC QGLW EVFV TLSE RARD YLHK TGRF IVIG GIVS PVHD SYGK QGLV SSRH RLIM CQLA VQNS DWIR VDPW ECYQ DTWQ TTCS VLEH HRDL MKRV TGCI LSNV NTPS MTPV IGQP QNET PQPI YQNS NVAT KPTA AKIL GKVG ESLS RICC VRPP VERF TFVD ENAN LGTV MRYE EIEL RILL LCGS DLLE SFCI PGLW NEAD MEVI VGDF GIVV VPRD AADT DRIM NHSS ILRK YKNN IMVV KDDI NHPM SVVS STKS RLAL QHGD GHVV DYLS QPVI DYIL KSQL YINA SG
Gene ID:23057
Uniprot:Q9BZQ4
Cellular Location:Cytoplasm, Golgi apparatus
Calculated MW:34kDa
Observed MW:37kDa
Synonyms:NMNAT2, C1orf15, PNAT2
Background:This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:NMNAT2: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Belongs to the eukaryotic NMN adenylyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.7.7.1; EC 2.7.7.18; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Transferase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; Golgi membrane

Molecular Function:nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity

Biological Process: NAD biosynthetic process; NAD metabolic process

NCBI Summary:This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BZQ4
NCBI GenInfo Identifier:30580486
NCBI Gene ID:23057
NCBI Accession:Q9BZQ4.1
UniProt Secondary Accession:Q9BZQ4,O75067, Q5T1Q3, Q8WU99, Q96QW1,
UniProt Related Accession:Q9BZQ4
Molecular Weight:34,015 Da
NCBI Full Name:Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
NCBI Synonym Full Names:nicotinamide nucleotide adenylyltransferase 2
NCBI Official Symbol:NMNAT2  
NCBI Official Synonym Symbols:PNAT2; C1orf15  
NCBI Protein Information:nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
UniProt Protein Name:Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
UniProt Synonym Protein Names:Nicotinamide mononucleotide adenylyltransferase 2; NMN adenylyltransferase 2; Nicotinate-nucleotide adenylyltransferase 2; NaMN adenylyltransferase 2
UniProt Gene Name:NMNAT2  
UniProt Entry Name:NMNA2_HUMAN
Anti-NMNAT2 Antibody (CAB13833)Western blot analysis of extracts of various cell lines, using NMNAT2 antibody (CAB13833) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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