Cell Biology Antibodies 1
Anti-NMI Antibody (CAB10265)
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- SKU:
- CAB10265
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NMI Antibody |
| Antibody SKU: | CAB10265 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human NMI (NP_004679.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-87MG, HepG2, SW480 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human NMI (NP_004679.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEAD KDDT QQIL KEHS PDEF IKDE QNKG LIDE ITKK NIQL KKEI QKLE TELQ EATK EFQI KEDI PETK MKFL SVET PEND SQLS NISC SFQV SSKV PYEI QKGQ ALIT FEKE EVAQ NVVS MSKH HVQI KDVN LEVT AKPV PLNS GVRF QVYV EVSK MKIN VTEI PDTL REDQ MRDK LELS FSKS RNGG GEVD RVDY DRQS GSAV ITFV EIGV ADKI LKKK EYPL YINQ TCHR VTVS PYTE IHLK KYQI FSGT SKRT VLLT GMEG IQMD EEIV EDLI NIHF QRAK NGGG EVDV VKCS LGQP HIAY FEE |
| Gene ID: | 9111 |
| Uniprot: | Q13287 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 35kDa |
| Observed MW: | 38kDa |
| Synonyms: | NMI |
| Background: | NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. |
| UniProt Protein Function: | NMI: May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5. Belongs to the NMI family. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 2q23.3 Cellular Component: cytoplasm; cytosol; nucleoplasm Molecular Function:identical protein binding; protein binding Biological Process: inflammatory response; JAK-STAT cascade |
| NCBI Summary: | NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13287 |
| NCBI GenInfo Identifier: | 116242679 |
| NCBI Gene ID: | 9111 |
| NCBI Accession: | Q13287.2 |
| UniProt Secondary Accession: | Q13287,Q53TI8, Q8WTW2, Q9BVE5, B5BU69, |
| UniProt Related Accession: | Q13287 |
| Molecular Weight: | 35kDa |
| NCBI Full Name: | N-myc-interactor |
| NCBI Synonym Full Names: | N-myc and STAT interactor |
| NCBI Official Symbol: | NMI |
| NCBI Protein Information: | N-myc-interactor |
| UniProt Protein Name: | N-myc-interactor |
| UniProt Synonym Protein Names: | N-myc and STAT interactor |
| Protein Family: | N-myc-interactor |
| UniProt Gene Name: | NMI |
 | Western blot analysis of extracts of various cell lines, using NMI antibody (CAB10265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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