Antibody Name:	Anti-NME2 Antibody
Antibody SKU:	CAB7443
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human NME2 (NP_001018148.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	PC-12, 22Rv1, Mouse kidney, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human NME2 (NP_001018148.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MANL ERTF IAIK PDGV QRGL VGEI IKRF EQKG FRLV AMKF LRAS EEHL KQHY IDLK DRPF FPGL VKYM NSGP VVAM VWEG LNVV KTGR VMLG ETNP ADSK PGTI RGDF CIQV GRNI IHGS DSVK SAEK EISL WFKP EELV DYKS CAHD WVYE
Gene ID:	4831
Uniprot:	P22392
Cellular Location:	Cell projection, Cytoplasm, Nucleus, lamellipodium, ruffle
Calculated MW:	17kDa/30kDa
Observed MW:	17kDa

Synonyms:

NME2, NDKB, NDPK-B, NDPKB, NM23-H2, NM23B, PUF

Background:

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product.

UniProt Protein Function:	NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - purine; EC 2.7.13.3; Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine; Kinase, nucleoside diphosphate; Kinase, other; EC 2.7.4.6; Other group; NDK family Chromosomal Location of Human Ortholog: 17q21.3 Cellular Component: cytoplasm; cytosol; focal adhesion; lamellipodium; nucleus; ruffle Molecular Function:nucleoside diphosphate kinase activity; protein binding; transcription factor activity Biological Process: cell adhesion; integrin-mediated signaling pathway; negative regulation of apoptosis; nucleobase, nucleoside and nucleotide interconversion; nucleoside triphosphate biosynthetic process; positive regulation of epithelial cell proliferation; positive regulation of keratinocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of epidermis development; regulation of transcription, DNA-dependent
NCBI Summary:	Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]
UniProt Code:	P22392
NCBI GenInfo Identifier:	127983
NCBI Gene ID:	4831
NCBI Accession:	P22392.1
UniProt Secondary Accession:	P22392,Q1WM23, Q6LCT6, A8MWA3,
UniProt Related Accession:	P22392
Molecular Weight:	30,137 Da
NCBI Full Name:	Nucleoside diphosphate kinase B
NCBI Synonym Full Names:	NME/NM23 nucleoside diphosphate kinase 2
NCBI Official Symbol:	NME2
NCBI Official Synonym Symbols:	PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2
NCBI Protein Information:	nucleoside diphosphate kinase B
UniProt Protein Name:	Nucleoside diphosphate kinase B
UniProt Synonym Protein Names:	C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB (EC:2.7.13.3); nm23-H2
UniProt Gene Name:	NME2
UniProt Entry Name:	NDKB_HUMAN

	Western blot analysis of extracts of various cell lines, using NME2 antibody (CAB7443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human kidney using NME2 antibody (CAB7443) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using NME2 antibody (CAB7443) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using NME2 antibody (CAB7443). Blue: DAPI for nuclear staining.