KO Validated Antibodies 1

Anti-NME1 Antibody (CAB17999)[KO Validated]

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SKU:
CAB17999
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-NME1 Antibody [KO Validated]
Antibody SKU:CAB17999
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 90 to the C-terminus of human NME1 (NP_000260.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 90 to the C-terminus of human NME1 (NP_000260.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLGE TNPA DSKP GTIR GDFC IQVG RNII HGSD SVES AEKE IGLW FHPE ELVD YTSC AQNW IYE
Gene ID:4830
Uniprot:P15531
Cellular Location:Cytoplasm, Nucleus
Calculated MW:17kDa/19kDa/30kDa
Observed MW:21kDa
Anti-NME1 Antibody [KO Validated] (CAB17999)Western blot analysis of extracts from normal (control) and NME1 knockout (KO) 293T cells, using NME1 antibody (CAB17999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:NME1, AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1
Background:This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
UniProt Protein Function:NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3'-5' exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported.
UniProt Protein Details:

Protein type:Kinase, other; EC 2.7.4.6; Tumor suppressor; Nucleotide Metabolism - pyrimidine; Kinase, nucleoside diphosphate; Protein kinase, histidine; Nucleotide Metabolism - purine; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: cytoplasm; cytosol; membrane; nucleus

Molecular Function:ATP binding; deoxyribonuclease activity; GTP binding; identical protein binding; magnesium ion binding; nucleoside diphosphate kinase activity; protein binding; ribosomal small subunit binding

Biological Process: negative regulation of cell proliferation; nucleobase, nucleoside and nucleotide interconversion; nucleoside triphosphate biosynthetic process; positive regulation of DNA binding; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of apoptosis

Disease: Neuroblastoma, Susceptibility To

NCBI Summary:This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]
UniProt Code:P15531
NCBI GenInfo Identifier:127981
NCBI Gene ID:4830
NCBI Accession:P15531.1
UniProt Secondary Accession:P15531,Q6FGK3, Q86XQ2, Q9UDJ6,
UniProt Related Accession:P15531
Molecular Weight:30,137 Da
NCBI Full Name:Nucleoside diphosphate kinase A
NCBI Synonym Full Names:NME/NM23 nucleoside diphosphate kinase 1
NCBI Official Symbol:NME1  
NCBI Official Synonym Symbols:NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1  
NCBI Protein Information:nucleoside diphosphate kinase A
UniProt Protein Name:Nucleoside diphosphate kinase A
UniProt Synonym Protein Names:Granzyme A-activated DNase; GAAD; Metastasis inhibition factor nm23; NM23-H1; Tumor metastatic process-associated protein
Protein Family:Nucleoside diphosphate kinase
UniProt Gene Name:NME1  
UniProt Entry Name:NDKA_HUMAN
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