Antibody Name:	NLRP2 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8233
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NLRP2 (NP_060322.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NLRP2 (NP_060322.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVSS AQMG FNLQ ALLE QLSQ DELS KFKY LITT FSLA HELQ KIPH KEVD KADG KQLV EILT THCD SYWV EMAS LQVF EKMH RMDL SERA KDEV REAA LKSF NKRK PLSL GITR KERP PLDV DEML ERFK TEAQ AFTE TKGN VICL GKEV FKGK KPDK DNRC RYIL KTKF REMW KSWP GDSK EVQV MAER YKML IPFS NPRV LPGP FSYT VVLY GPAG LGKT
Gene ID:	55655
Uniprot:	Q9NX02
Cellular Location:	Cytoplasm
Calculated MW:	96kDa/117kDa/118kDa/120kDa
Observed MW:	121kDa

Synonyms:

NLRP2, CLR19.9, NALP2, NBS1, PAN1, PYPAF2

Background:

This gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). Members of this gene family are thought to be important regulators of immune responses. This gene product interacts with components of the IkB kinase (IKK) complex, and can regulate both caspase-1 and NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) activity. The pyrin domain is necessary and sufficient for suppression of NF-kB activity. An allelic variant (rs147585490) has been found that is incapable of blocking the transcriptional activity of NF-kB. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	NLRP2: Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 19q13.42 Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; nuclear chromosome, telomeric region Molecular Function:protein binding; Pyrin domain binding Biological Process: positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion
NCBI Summary:	This gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). Members of this gene family are thought to be important regulators of immune responses. This gene product interacts with components of the IkB kinase (IKK) complex, and can regulate both caspase-1 and NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) activity. The pyrin domain is necessary and sufficient for suppression of NF-kB activity. An allelic variant (rs147585490) has been found that is incapable of blocking the transcriptional activity of NF-kB. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]
UniProt Code:	Q9NX02
NCBI GenInfo Identifier:	17380148
NCBI Gene ID:	55655
NCBI Accession:	Q9NX02.1
UniProt Secondary Accession:	Q9NX02,Q53FL5, Q59G09, Q8IXT0, Q9BVN5, Q9H6G6, Q9HAV9 Q9NWK3, B4DZL7, I3L0G4,
UniProt Related Accession:	Q9NX02
Molecular Weight:	117,883 Da
NCBI Full Name:	NACHT, LRR and PYD domains-containing protein 2
NCBI Synonym Full Names:	NLR family pyrin domain containing 2
NCBI Official Symbol:	NLRP2
NCBI Official Synonym Symbols:	NBS1; PAN1; NALP2; PYPAF2; CLR19.9
NCBI Protein Information:	NACHT, LRR and PYD domains-containing protein 2
UniProt Protein Name:	NACHT, LRR and PYD domains-containing protein 2
UniProt Synonym Protein Names:	Nucleotide-binding site protein 1; PYRIN domain and NACHT domain-containing protein 1; PYRIN-containing APAF1-like protein 2
UniProt Gene Name:	NLRP2

	Western blot analysis of extracts of A-549 cells, using NLRP2 antibody (CAB8233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using NLRP2 antibody (CAB8233) at dilution of 1:100. Blue: DAPI for nuclear staining.