Name: Anti-NLRP12 Antibody (CAB14519)
Brand: Cell Biology Antibodies 5
SKU: CAB14519
Availability: OutOfStock

Antibody Name:	Anti-NLRP12 Antibody
Antibody SKU:	CAB14519
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 862-1062 of human NLRP12 (NP_001264055.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Rat
Positive Samples:	Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 862-1062 of human NLRP12 (NP_001264055.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LWLK ICRL TAAA CDEL ASTL SVNQ SLRE LDLS LNEL GDLG VLLL CEGL RHPT CKLQ TLRL GICR LGSA ACEG LSVV LQAN HNLR ELDL SFND LGDW GLWL LAEG LQHP ACRL QKLW LDSC GLTA KACE NLYF TLGI NQTL TDLY LTNN ALGD TGVR LLCK RLSH PGCK LRVL WLFG MDLN KMTH SRLA ALRV TKPY LDIG C
Gene ID:	91662
Uniprot:	P59046
Cellular Location:	Cytoplasm
Calculated MW:	31kDa/101kDa/107kDa/113kDa/114kDa/120kDa
Observed MW:	120kDa

Synonyms:

NLRP12, CLR19.3, FCAS2, NALP12, PAN6, PYPAF7, RNO, RNO2

Background:

This gene encodes a member of the CATERPILLER family of cytoplasmic proteins. The encoded protein, which contains an N-terminal pyrin domain, a NACHT domain, a NACHT-associated domain, and a C-terminus leucine-rich repeat region, functions as an attenuating factor of inflammation by suppressing inflammatory responses in activated monocytes. Mutations in this gene cause familial cold autoinflammatory syndrome type 2. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NLRP12: May mediate activation of CASP1 via ASC and promote activation of NF-kappa-B via IKK. Defects in NLRP12 are the cause of familial cold autoinflammatory syndrome type 2 (FCAS2). FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 19q13.42 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding Biological Process: inhibition of NF-kappaB transcription factor; negative regulation of cytokine secretion; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of inflammatory response; negative regulation of interleukin-1 secretion; negative regulation of interleukin-6 biosynthetic process; negative regulation of protein amino acid autophosphorylation; negative regulation of signal transduction; negative regulation of Toll signaling pathway; positive regulation of MHC class I biosynthetic process; regulation of caspase activity; regulation of I-kappaB kinase/NF-kappaB cascade; release of cytoplasmic sequestered NF-kappaB Disease: Familial Cold Autoinflammatory Syndrome 2
NCBI Summary:	This gene encodes a member of the CATERPILLER family of cytoplasmic proteins. The encoded protein, which contains an N-terminal pyrin domain, a NACHT domain, a NACHT-associated domain, and a C-terminus leucine-rich repeat region, functions as an attenuating factor of inflammation by suppressing inflammatory responses in activated monocytes. Mutations in this gene cause familial cold autoinflammatory syndrome type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
UniProt Code:	P59046
NCBI GenInfo Identifier:	34223733
NCBI Gene ID:	91662
NCBI Accession:	P59046.2
UniProt Secondary Accession:	P59046,Q8NEU4, Q9BY26, A8MTQ2, B3KTE7,
UniProt Related Accession:	P59046
Molecular Weight:	120,329 Da
NCBI Full Name:	NACHT, LRR and PYD domains-containing protein 12
NCBI Synonym Full Names:	NLR family pyrin domain containing 12
NCBI Official Symbol:	NLRP12
NCBI Official Synonym Symbols:	RNO; PAN6; RNO2; FCAS2; NALP12; PYPAF7; CLR19.3
NCBI Protein Information:	NACHT, LRR and PYD domains-containing protein 12
UniProt Protein Name:	NACHT, LRR and PYD domains-containing protein 12
UniProt Synonym Protein Names:	Monarch-1; PYRIN-containing APAF1-like protein 7; Regulated by nitric oxide
Protein Family:	NACHT, LRR and PYD domains-containing protein
UniProt Gene Name:	NLRP12
UniProt Entry Name:	NAL12_HUMAN

Western blot analysis of extracts of rat spleen, using NLRP12 antibody (CAB14519) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-NLRP12 Antibody (CAB6671)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)