Anti-Nitrotyrosine Antibody [39B6]

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SKU:
STMA00033
Antibody Type:
Monoclonal
Applications:
WB
Applications:
IHC
Applications:
ICC/IF
Applications:
IP
Applications:
ELISA
Applications:
FCM
Applications:
AM
Reactivity:
Species Independent
Host Species:
Mouse
Isotype:
IgG2a
  • Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (STMA00033). Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (STMA00033) at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.
  • Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (STMA00033). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (STMA00033) at 1:1000000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.
  • Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (STMA00033). Tissue: liver tissue. Species: Rat. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (STMA00033) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green).
  • Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (STMA00033). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (STMA00033) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.
  • Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (STMA00033). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (STMA00033) at 1:25000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.
  • Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosine protein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (STMA00033). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (STMA00033) at 1:1000 for 2.5 hours at RT with shaking.
  • Western Blot analysis of Human Recombinant Protein showing detection of Multiple Bands Nitrotyrosine protein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (STMA00033). Lane 1: MW Ladder. Lane 2: hASYN Monomer (3.84 ug). Lane 3: Nitrosylated hASYN (3.84 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (STMA00033) at 1:1000 for 2 hours at RT with shaking.
  • Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosine protein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (STMA00033). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (STMA00033) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking.
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Description

Nitrotyrosine Antibody [39B6]

Nitrotyrosine Antibody [39B6] is a mouse monoclonal antibody directed against Nitrotyrosine, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF, IP, ELISA, FCM and AM. Reactivity is species independent. Supplied as IgG2a, purified by Protein G.

Nitrotyrosine is a post-translational modification formed by the nitration of tyrosine residues, often mediated by reactive nitrogen species such as peroxynitrite. It serves as a biomarker of nitrosative stress and is increasingly recognized for its role in neurodegenerative disease pathology. Nitrotyrosine-modified proteins are frequently detected in Alzheimer’s disease, Parkinson’s disease, and ALS, where they correlate with inflammation-induced tissue injury. Enzymes such as myeloperoxidase, cytochrome P450s, and superoxide dismutase catalyze tyrosine nitration, disrupting protein function and contributing to neuronal damage. As a marker of oxidative and nitrosative stress, nitrotyrosine provides mechanistic insight into redox imbalance and may serve as a diagnostic and therapeutic target in neurodegeneration. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:Nitrotyrosine Antibody [39B6]
Product SKU:STMA00033
Size:100 µg
Target:Nitrotyrosine
Host Species:Mouse
Antibody Type:Monoclonal
Clone ID:39B6
Research Areas:Cancer, Oxidative Stress, Cell Signaling, Post-translational Modifications, Nitration, Neuroscience, Neurodegeneration, Alzheimer's Disease, Parkinson's Disease
Buffer:PBS, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, IHC, ICC/IF, IP, ELISA, FCM, AM
Antibody Isotype:IgG2a
Purification:Protein G Purified
Concentration:1 mg/ml
Reactivity:Species Independent
Recommended Dilution:WB (1:1400), IHC (1:100); optimal dilutions for assays should be determined by the user.
Specificity:Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
Guarantee:12 months from date of dispatch
Immunogen:3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
Alternative Names:Nitrotyrosine, Nitro tyrosine, 3-Nitrotyrosine
References:1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8. 3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330(2): 795-801.
Note:For research use only.
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