Name: Anti-NHEJ1 Antibody (CAB19957)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19957
Availability: OutOfStock

Antibody Name:	Anti-NHEJ1 Antibody [KO Validated]
Antibody SKU:	CAB19957
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human NHEJ1.

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant protein of human NHEJ1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	79840
Uniprot:	Q9H9Q4
Cellular Location:	Nucleus
Calculated MW:	33kDa/35kDa
Observed MW:	39KDa

Anti-NHEJ1 Antibody [KO Validated] (CAB19957)

Western blot analysis of extracts from normal (control) and NHEJ1 knockout (KO) HeLa cells, using NHEJ1 antibody (CAB19957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.

Synonyms:	NHEJ1, XLF
Background:	Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

UniProt Protein Function:	NHEJ1: DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Interacts with XRCC4 and the XRCC4-LIG4 complex. Binds DNA in a length-dependent manner. Ubiquitously expressed. Belongs to the XLF family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 2q35 Cellular Component: nucleus Molecular Function:protein binding; DNA binding Biological Process: central nervous system development; B cell differentiation; response to ionizing radiation; positive regulation of ligase activity; double-strand break repair via nonhomologous end joining; DNA recombination; T cell differentiation Disease: Severe Combined Immunodeficiency With Microcephaly, Growth Retardation, And Sensitivity To Ionizing Radiation
NCBI Summary:	Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H9Q4
NCBI GenInfo Identifier:	74734059
NCBI Gene ID:	79840
NCBI Accession:	Q9H9Q4.1
UniProt Secondary Accession:	Q9H9Q4,Q4ZFW7, Q6IA64, Q96JS9, B8ZZA4,
UniProt Related Accession:	Q9H9Q4
Molecular Weight:	299
NCBI Full Name:	Non-homologous end-joining factor 1
NCBI Synonym Full Names:	nonhomologous end-joining factor 1
NCBI Official Symbol:	NHEJ1
NCBI Official Synonym Symbols:	XLF
NCBI Protein Information:	non-homologous end-joining factor 1; XRCC4-like factor; protein cernunnos
UniProt Protein Name:	Non-homologous end-joining factor 1
UniProt Synonym Protein Names:	Protein cernunnos; XRCC4-like factor
Protein Family:	Non-homologous end-joining factor
UniProt Gene Name:	NHEJ1
UniProt Entry Name:	NHEJ1_HUMAN