Epigenetics & Nuclear Signaling Antibodies 4

Anti-NFATC3 Antibody (CAB6666)

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SKU:
CAB6666
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NFATC3 Antibody
Antibody SKU:CAB6666
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human NFATC3 (NP_775188.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human NFATC3 (NP_775188.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTTA NCGA HDEL DFKL VFGE DGAP APPP PGSR PADL EPDD CASI YIFN VDPP PSTL TTPL CLPH HGLP SHSS VLSP SFQL QSHK NYEG TCEI PESK YSPL GGPK PFEC PSIQ ITSI SPNC HQEL DAHE DDLQ INDP EREF LERP SRDH LYLP LEPS YRES SLSP SPAS SISS RSWF SDAS SCES LSHI YDDV DSEL NEAA ARFT LGSP LTSP GGSP GGCP GEET WHQQ YGLG HSLS PRQS PCHS PRSS VTDE NWLS PRPA SGPS SRPT SPCG KRRH SSAE VCYA GSLS PH
Gene ID:4775
Uniprot:Q12968
Cellular Location:Cytoplasm, Nucleus
Calculated MW:77kDa/80kDa/112kDa/115kDa
Observed MW:150kDa
Synonyms:NFATC3, NFAT4, NFATX
Background:The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene.
UniProt Protein Function:NFAT4: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells. Plays a role in the regulation of gene expression in T cells and immature thymocytes. Six spliced isoforms have been identified. Isoform 1 is predominantly expressed in thymus, peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function:protein binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; heart development; cellular respiration; muscle cell development; innate immune response; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; inflammatory response

NCBI Summary:The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010]
UniProt Code:Q12968
NCBI GenInfo Identifier:9087155
NCBI Gene ID:4775
NCBI Accession:Q12968.1
UniProt Secondary Accession:Q12968,O75211, Q14516, Q99840, Q99841, Q99842,
UniProt Related Accession:Q12968
Molecular Weight:1075
NCBI Full Name:Nuclear factor of activated T-cells, cytoplasmic 3
NCBI Synonym Full Names:nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
NCBI Official Symbol:NFATC3  
NCBI Official Synonym Symbols:NFAT4; NFATX  
NCBI Protein Information:nuclear factor of activated T-cells, cytoplasmic 3; NF-ATc3; T cell transcription factor NFAT4; nuclear factor of activated T-cells c3 isoform IE-Xa
UniProt Protein Name:Nuclear factor of activated T-cells, cytoplasmic 3
UniProt Synonym Protein Names:NFATx; T-cell transcription factor NFAT4; NF-AT4
Protein Family:Nuclear factor of activated T-cells
UniProt Gene Name:NFATC3  
UniProt Entry Name:NFAC3_HUMAN
Anti-NFATC3 Antibody (CAB6666)Western blot analysis of extracts of various cell lines, using NFATC3 antibody (CAB6666) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-NFATC3 Antibody (CAB6666)Immunohistochemistry of paraffin-embedded human colon carcinoma using NFATC3 Rabbit pAb (CAB6666) at dilution of 1:200 (40x lens).
Anti-NFATC3 Antibody (CAB6666)Immunofluorescence analysis of HeLa cells using NFATC3 antibody (CAB6666). Blue: DAPI for nuclear staining.
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