Antibody Name:	Anti-NFATC2 Antibody
Antibody SKU:	CAB3107
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human NFATC2

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:1000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	K562, mouse intestine

Immunogen:	A synthetic peptide of human NFATC2
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4773
Uniprot:	Q13469
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	76kDa/97kDa/98kDa/99kDa/100kDa
Observed MW:	130kDa

Synonyms:

NFATC2, NFAT1, NFATP

Background:

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized.

UniProt Protein Function:	NFAT1: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. It is present in a phosphorylated the cytosol of unstimulated T cells. After T-cell activation, it is dephosphorylated by calcineurin and translocates to the nucleus as a part of a transcription complex. Two splice variant isoforms have been identified.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 20q13.2 Cellular Component: nucleoplasm; cytoplasm; plasma membrane; ribonucleoprotein complex; nucleus; cytosol; actin cytoskeleton Molecular Function:protein binding; DNA binding; transcription factor activity Biological Process: response to drug; B cell receptor signaling pathway; cell migration; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; cytokine production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus
NCBI Summary:	This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]
UniProt Code:	Q13469
NCBI GenInfo Identifier:	68846905
NCBI Gene ID:	4773
NCBI Accession:	Q13469.2
UniProt Secondary Accession:	Q13469,Q13468, Q5TFW7, Q5TFW8, Q9NPX6, Q9NQH3, Q9UJR2 B5B2N8, B5B2N9, B5B2P0, B5B2P2, B5B2P3,
UniProt Related Accession:	Q13469
Molecular Weight:	901
NCBI Full Name:	Nuclear factor of activated T-cells, cytoplasmic 2
NCBI Synonym Full Names:	nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
NCBI Official Symbol:	NFATC2
NCBI Official Synonym Symbols:	NFAT1; NFATP
NCBI Protein Information:	nuclear factor of activated T-cells, cytoplasmic 2; NF-ATc2; NFAT pre-existing subunit; T cell transcription factor NFAT1; T-cell transcription factor NFAT1; NFAT transcription complex, preexisting component; preexisting nuclear factor of activated T-cells 2; nuclear factor of activated T-cells, preexisting component
UniProt Protein Name:	Nuclear factor of activated T-cells, cytoplasmic 2
UniProt Synonym Protein Names:	NFAT pre-existing subunit; NF-ATp; T-cell transcription factor NFAT1
Protein Family:	NFATC2-interacting protein
UniProt Gene Name:	NFATC2
UniProt Entry Name:	NFAC2_HUMAN

	Western blot analysis of extracts of various cell lines, using NFATC2 antibody (CAB3107) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of Jurkat cells using NFATC2 Rabbit pAb (CAB3107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of rat spleen using NFATC2 Rabbit pAb (CAB3107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.